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Product Info Summary
| SKU: | A04282-3 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Human, Mouse, Pig, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-CACNA2D1 Antibody
SKU/Catalog Number
A04282-3
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-CACNA2D1 Antibody catalog # A04282-3. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat, Pig.
Storage & Handling
12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
Cite This Product
Anti-CACNA2D1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04282-3)
Host
Rabbit
Contents
500 μg/ml antibody with PBS, 0.02% NaN3, 1 mg stabilizing protein and 50% glycerol
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Immunogen
E.coli-derived human CACNA2D1 recombinant protein (Position: P27-E121).
Reactive Species
A04282-3 is reactive to CACNA2D1 in Human, Mouse, Pig, Rat
Calculated molecular weight
124.6 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04282-3 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Recommend Dilution
| Application | Dilution | Species |
|---|---|---|
| Western Blot (WB) | 1:500-2000 | |
| Immunohistochemistry (IHC) | 1:50-400 | |
| Immunofluorescence (IF) | 1:50-400 | |
| ELISA | 1:100-1000 |
Validation Images & Assay Conditions
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Western blot analysis of CACNA2D1 using anti-CACNA2D1 antibody (A04282-3).
Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat skeletal muscle tissue lysates,
Lane 2: rat heart tissue lysates,
Lane 3: mouse skeletal muscle tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CACNA2D1 antigen affinity purified polyclonal antibody (A04282-3) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for CACNA2D1 at approximately 140 kDa. The expected band size for CACNA2D1 is at 125 kDa.
Specific Publications For Anti-CACNA2D1 Antibody (A04282-3)
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