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Product Info Summary
|Reactive Species:||Human, Mouse|
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Anti-Cadherin 16 CDH16 Monoclonal Antibody
Boster Bio Anti-Cadherin 16 CDH16 Monoclonal Antibody catalog # M07518-1. Tested in IP, WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cadherin 16 CDH16 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M07518-1)
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
A synthesized peptide derived from human Cadherin 16 Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types.
M07518-1 is reactive to CDH16 in Human, Mouse
M07518-1 is guaranteed for IP, WB Boster Guarantee
*Blocking peptide can be purchased at $150. Contact us for more information.
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
WB 1:500~1:2000 IHC 1:100~1:500 IP 1:40
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of CDH16 using anti-CDH16 antibody (M07518-1) in Human fetal kidney lysate..
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDH16 antigen affinity purified polyclonal antibody (Catalog # M07518-1) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system.
Gene/Protein Information For CDH16 (Source: Uniprot.Org, NCBI)
cadherin 16, KSP-cadherin; cadherin-16; Kidney-specific cadherin; KSP-cadherin CDH16 cadherin 16 cadherin-16|KSP-cadherin|cadherin 16, KSP-cadherin|kidney-specific cadherin*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on CDH16, check out the CDH16 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CDH16: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
No publications found for M07518-1
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