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Product Info Summary
| SKU: | A14851 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, WB |
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Product info
Product Name
Anti-Cadherin-26 Antibody
SKU/Catalog Number
A14851
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Cadherin-26 Antibody catalog # A14851. Tested in WB, IF, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cadherin-26 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14851)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human CDH26. AA range:11-60
Reactive Species
A14851 is reactive to CDH26 in Human, Mouse
Calculated molecular weight
92.4 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A14851 is guaranteed for ELISA, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IF 1:200-1:1000
ELISA 1:10000
Validation Images & Assay Conditions
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Western Blot analysis of various cells using Cadherin-26 Polyclonal Antibody
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Immunofluorescence analysis of MCF7 cells, using CDH26 Antibody. The picture on the right is blocked with the synthesized peptide.
Specific Publications For Anti-Cadherin-26 Antibody (A14851)
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7 Customer Q&As for Anti-Cadherin-26 Antibody
Question
Will anti-Cadherin-26 antibody A14851 work for WB with lung tonsillar carcinoma?
Verified Customer
Verified customer
Asked: 2020-05-08
Answer
According to the expression profile of lung tonsillar carcinoma, CDH26 is highly expressed in lung tonsillar carcinoma. So, it is likely that anti-Cadherin-26 antibody A14851 will work for WB with lung tonsillar carcinoma.
Boster Scientific Support
Answered: 2020-05-08
Question
Here is the WB image, lot number and protocol we used for lung tonsillar carcinoma using anti-Cadherin-26 antibody A14851. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-03-20
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-20
Question
Is this A14851 anti-Cadherin-26 antibody reactive to the isotypes of CDH26?
Verified Customer
Verified customer
Asked: 2020-03-02
Answer
The immunogen of A14851 anti-Cadherin-26 antibody is Synthesized peptide derived from the N-terminal region of human Cadherin-26. at AA rangle: 30-110. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-03-02
Question
Can you help my question with product A14851, anti-Cadherin-26 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-08-23
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A14851 anti-Cadherin-26 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-08-23
Question
We are currently using anti-Cadherin-26 antibody A14851 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2019-06-11
Answer
The anti-Cadherin-26 antibody (A14851) has not been validated for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-06-11
Question
Is a blocking peptide available for product anti-Cadherin-26 antibody (A14851)?
Verified Customer
Verified customer
Asked: 2017-05-24
Answer
We do provide the blocking peptide for product anti-Cadherin-26 antibody (A14851). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2017-05-24
Question
Would A14851 anti-Cadherin-26 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
S. Evans
Verified customer
Asked: 2017-02-23
Answer
As indicated on the product datasheet, A14851 anti-Cadherin-26 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-02-23
