Anti-Calcipressin-2 RCAN2 Antibody

Product Name

Anti-Calcipressin-2 RCAN2 Antibody

View all RCAN2 Antibodies

SKU/Catalog Number

A11369

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Calcipressin-2 RCAN2 Antibody (Catalog # A11369). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

RCAN2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-Calcipressin-2 RCAN2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11369)

Host

Rabbit

Contents

RCAN2 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

RCAN2 antibody was raised against a 14 amino acid synthetic peptide near the center of human RCAN2. The immunogen is located within amino acids 70 - 120 of RCAN2.

Reactive Species

A11369 is reactive to RCAN2 in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

22.0 kDa

Background of RCAN2

Regulator of calcineurin 2 (RCAN2), also known as ZAKI4 and DSCR1L1, is expressed as two isoforms differing at their N-terminus. The longer of the two (isoform 1) is expressed exclusively in the brain, while isoform 2 is ubiquitously expressed, with highest expression in brain, heart, and muscle. Both isoforms bind to the catalytic subunit of calcineurin, a Ca++-dependent protein phosphatase involved in several neuronal functions, though their C-terminal region and inhibit calcineurin's activity. Unlike isoform 1 of RCAN2, the expression of the second isoform is not induced by the thyroid hormone T3. RCAN2 is a member of a family of three endogenous calcineurin regulators that are located near the minimal supernumerary fragment of chromosome 21 in individuals with Down syndrome, suggesting that they play a role in this syndrome. Multiple isoforms of RCAN2 are known to exist.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A11369 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

RCAN2 antibody can be used for detection of RCAN2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information RCAN2

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