Anti-Caldesmon (Smooth) Antibody (Monoclonal, hHCD)

SKU MA1009
Size 100μg/vial
Reactivity Human, Mouse, Rabbit
Clonality Monoclonal
Clone Number hHCD
Host Mouse
Ig Isotype Mouse IgG1
Applications IHC, WB

Overview

Product Name Anti-Caldesmon (Smooth) Antibody (Monoclonal, hHCD)
SKU/Catalog Number MA1009
Storage & Handling At -20°C for one year, at 4°C for one month. Avoid repeated freezing and thawing.
Size 100μg/vial
Description Mouse IgG monoclonal antibody for Caldesmon (smooth), caldesmon 1 (CALD1) detection. Tested with WB, IHC-P in Human;mouse;rabbit. No cross reactivity with other proteins.
Cite This Product Anti-Caldesmon (Smooth) Antibody (Monoclonal, hHCD) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1009)
Host Mouse
Contents/Buffer Each vial contains 50% glycerol, 1.2% Sodium acetate, 1% BSA, 0.02% NaN3.
Form Liquid
Immunogen Human uterus smooth muscle extract.
Reactivity Human, Mouse, Rabbit

Assay Details

Assay Dilutions Overview

Concentration: 0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, mouse, rabbit, By Heat
Western blot, 0.25-0.5μg/ml, Human, mouse, rabbit

Boster's Secondary Antibodies And IHC, WB Kits

The following reagents are used to generate the images below.

Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P).

Images And Assay Conditions

Anti-Caldesmon(smooth) antibody, MA1009, IHC(P)
IHC(P): Human Mammary Cancer Tissue

Anti-Caldesmon(smooth) antibody, MA1009, IHC(P)
IHC(P): Human Intestinal Cancer Tissue

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id Q62736
Gene Name Cald1
Protein Name Non-muscle caldesmon
Tissue Specificity High-molecular-weight caldesmon (h-caldesmon) is predominantly expressed in smooth muscles, whereas low- molecular-weight caldesmon (l-caldesmon) is widely distributed in non-muscle tissues and cells. Not expressed in skeletal muscle or heart.
Alternative Names Non-muscle caldesmon;CDM;L-caldesmon;Cald1;
Subcellular Localization Cytoplasm, cytoskeleton . Cytoplasm, myofibril . On thin filaments in smooth muscle and on stress fibers in fibroblasts (nonmuscle). .
Molecular Weight 60584 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Actin- and myosin-binding protein implicated in the regulation of actomyosin interactions in smooth muscle and nonmuscle cells (could act as a bridge between myosin and actin filaments). Stimulates actin binding of tropomyosin which increases the stabilization of actin filament structure. In muscle tissues, inhibits the actomyosin ATPase by binding to F-actin. This inhibition is attenuated by calcium-calmodulin and is potentiated by tropomyosin. Interacts with actin, myosin, two molecules of tropomyosin and with calmodulin. Also play an essential role during cellular mitosis and receptor capping. Involved in Schwann cell migration during peripheral nerve regeneration. .
Research Areas Human, Mouse, Rabbit

*You can search these to find other products in these research areas.
Background Caldesmon is a potential actomyosin regulatory protein found in smooth muscle and nonmuscle cells. The predicted smooth-muscle polypeptide is 793 amino acids long. The high molecular weight caldesmon(h-CaD) is predominantly expressed in smooth muscles, whereas the low molecular weight caldesmon(l-CaD) is widely distributed in nonmuscle tissues and cells. Hayashi et al.(1992) demonstrated that the human CDM gene is composed of 14 exons.

Other Recommended Resources

Here are featured tools and databases that you might find useful.

Monoclonal clone# G2 antibody for Caldesmon/CALD1 detection. Host: Mouse.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. Caldesmon/CALD1 information: Molecular Weight: 60584 MW; Subcellular Localization: Cytoplasm, cytoskeleton . Cytoplasm, myofibril . On thin filaments in smooth muscle and on stress fibers in fibroblasts (nonmuscle); Tissue Specificity: High-molecular-weight caldesmon (h-caldesmon) is predominantly expressed in smooth muscles, whereas low- molecular-weight caldesmon (l-caldesmon) is widely distributed in non-muscle tissues and cells. Not expressed in skeletal muscle or heart.
Write Your Own Review
Only registered users can write reviews. Please Sign in or create an account
In stock
Order Product
MA1009
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$280.00

Troubleshooting

troubleshooting_box_image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

We promise all of our products perform as described in datasheets.

Customer Q&As

  • Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
    A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
  • Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
    A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
  • Q: Can you provide monoclonal antibodies carrier free?
    A: For most of our monoclonal antibodies, we are unable to provide them carrier free. If you would like to check if a specific antibody can be provided carrier free, please send an email to support@bosterbio.com.
  • Q: What are some alternative names that could be used to describe this product?
    A: One other very common name is caldesmon antibody
Write a review for MA1009