radio
SKU MA1009
Size 100μg/vial
Reactivity Human, Mouse, Rabbit
Clonality Monoclonal
Clone Number hHCD
Host Mouse
Ig Isotype Mouse IgG1
Applications IHC, WB

Overview

Product Name Anti-Caldesmon (Smooth) Antibody (Monoclonal, hHCD)
SKU/Catalog Number MA1009
Storage & Handling At -20°C for one year, at 4°C for one month. Avoid repeated freezing and thawing.
Size 100μg/vial
Description Mouse IgG monoclonal antibody for Caldesmon (smooth), caldesmon 1 (CALD1) detection. Tested with WB, IHC-P in Human;mouse;rabbit. No cross reactivity with other proteins.
Cite This Product Anti-Caldesmon (Smooth) Antibody (Monoclonal, hHCD) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1009)
Host Mouse
Immunogen Human uterus smooth muscle extract.
Reactivity Human, Mouse, Rabbit

Assay Details

Assay Dilutions Overview

Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, mouse, rabbit, By Heat
Western blot, 0.25-0.5μg/ml, Human, mouse, rabbit

Boster's Secondary Antibodies And IHC, WB Kits

The following reagents are used to generate the images below.

Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P).

Images And Assay Conditions

/antibody/ma1009 1 IHC anti caldesmon smooth antibody monoclonal hhcd.jpg

Anti-Caldesmon(smooth) antibody, MA1009, IHC(P)
IHC(P): Human Intestinal Cancer Tissue

/antibody/ma1009 2 IHC anti caldesmon smooth antibody monoclonal hhcd.jpg

Anti-Caldesmon(smooth) antibody, MA1009, IHC(P)
IHC(P): Human Mammary Cancer Tissue

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id Q62736
Gene Name Cald1
Protein Name Non-muscle caldesmon
Tissue Specificity High-molecular-weight caldesmon (h-caldesmon) is predominantly expressed in smooth muscles, whereas low- molecular-weight caldesmon (l-caldesmon) is widely distributed in non-muscle tissues and cells. Not expressed in skeletal muscle or heart.
Alternative Names Non-muscle caldesmon;CDM;L-caldesmon;Cald1;
Subcellular Localization Cytoplasm, cytoskeleton . Cytoplasm, myofibril . On thin filaments in smooth muscle and on stress fibers in fibroblasts (nonmuscle). .
Molecular Weight 60584 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Actin- and myosin-binding protein implicated in the regulation of actomyosin interactions in smooth muscle and nonmuscle cells (could act as a bridge between myosin and actin filaments). Stimulates actin binding of tropomyosin which increases the stabilization of actin filament structure. In muscle tissues, inhibits the actomyosin ATPase by binding to F-actin. This inhibition is attenuated by calcium-calmodulin and is potentiated by tropomyosin. Interacts with actin, myosin, two molecules of tropomyosin and with calmodulin. Also play an essential role during cellular mitosis and receptor capping. Involved in Schwann cell migration during peripheral nerve regeneration. .
Background Caldesmon is a potential actomyosin regulatory protein found in smooth muscle and nonmuscle cells. The predicted smooth-muscle polypeptide is 793 amino acids long. The high molecular weight caldesmon(h-CaD) is predominantly expressed in smooth muscles, whereas the low molecular weight caldesmon(l-CaD) is widely distributed in nonmuscle tissues and cells. Hayashi et al.(1992) demonstrated that the human CDM gene is composed of 14 exons.

Order Product (MA1009)

Promotion:

Buy primary get secondary antibody for free.
Option Price
30ug sample size $99
100ug $280
100ug+Free HRP Secondary BA1050 $280
100ug+Free Biotin Secondary BA1001 $280
Ships in 5-7 business days.

Troubleshooting

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Customer Q&As

Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.
Q: What are some alternative names that could be used to describe this product?
A: One other very common name is caldesmon antibody