Anti-Caldesmon (Smooth) Cald1 Antibody (Monoclonal, hHCD)
Mouse IgG monoclonal antibody for Caldesmon (smooth), caldesmon 1 (CALD1) detection. Tested with WB, IHC-P in Human;mouse;rabbit. No cross reactivity with other proteins.
Anti-Caldesmon (Smooth) Cald1 Antibody (Monoclonal, hHCD) Info At A Glance
|Reactivity:||Human, Mouse, Rabbit|
|Product Name||Anti-Caldesmon (Smooth) Cald1 Antibody (Monoclonal, hHCD)
See all Caldesmon/CALD1 primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year, at 4°C for one month. Avoid repeated freezing and thawing.|
|Description||Monoclonal clone# G2 antibody for Caldesmon/CALD1 detection. Host: Mouse.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. Caldesmon/CALD1 information: Molecular Weight: 60584 MW; Subcellular Localization: Cytoplasm, cytoskeleton . Cytoplasm, myofibril . On thin filaments in smooth muscle and on stress fibers in fibroblasts (nonmuscle); Tissue Specificity: High-molecular-weight caldesmon (h-caldesmon) is predominantly expressed in smooth muscles, whereas low- molecular-weight caldesmon (l-caldesmon) is widely distributed in non-muscle tissues and cells. Not expressed in skeletal muscle or heart.|
|Cite This Product||Anti-Caldesmon (Smooth) Cald1 Antibody (Monoclonal, hHCD) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1009)|
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|Anti-Caldesmon (Smooth) Cald1 Antibody (Monoclonal, hHCD) may replace the following items: sc 12930 R|sc 12931|sc 12931 R|sc 135965|sc 15374|sc 25339|sc 271222|sc 48427|sc 58700|sc 58703|sc 7574|sc 7575|sc 7575 R.|
|Reactivity/Species||Human, Mouse, Rabbit|
*Our Boster Guarantee covers the use of this product in the above tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products.
|Related Products||Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round.
|Immunogen||Human uterus smooth muscle extract.|
|Cross Reactivity||No cross reactivity with other proteins|
Gene/Protein Basic Information For Cald1 (Source: Uniprot.org, NCBI)
|NCBI Gene Id||25687|
|Species Of This Entry||Rat|
|Protein Name||Non-muscle caldesmon|
|Alternative Names||Caldesmon/CALD1|CAD; CALD1; caldesmon 1; Caldesmon; CDM; CDMH-CAD; HCAD; LCAD; L-CAD; MGC21352; NAG22|
|Post Tranlational Modifications||Phosphorylation|
|Gene Location||4q22, on Chromasome 4, gene sequence:|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For Cald1 (Source: Uniprot.org, NCBI)
|Protein Function||Actin- and myosin-binding protein implicated in the regulation of actomyosin interactions in smooth muscle and nonmuscle cells (could act as a bridge between myosin and actin filaments). Stimulates actin binding of tropomyosin which increases the stabilization of actin filament structure. In muscle tissues, inhibits the actomyosin ATPase by binding to F-actin. This inhibition is attenuated by calcium-calmodulin and is potentiated by tropomyosin. Interacts with actin, myosin, two molecules of tropomyosin and with calmodulin. Also play an essential role during cellular mitosis and receptor capping. Involved in Schwann cell migration during peripheral nerve regeneration.|
|Tissue Specificity||High-molecular-weight caldesmon (h-caldesmon) is predominantly expressed in smooth muscles, whereas low- molecular-weight caldesmon (l-caldesmon) is widely distributed in non-muscle tissues and cells. Not expressed in skeletal muscle or heart.|
|Research Areas||Cell Biology; Cellular Markers|
|Related Diseases||Malignant Neoplasms; Neoplasms; Colonic Neoplasms; Malignant Neoplasm Of Prostate; Tissue Adhesions; Prostatic Neoplasms; Adenoma; Stress Fractures; Malignant Tumor Of Colon; Glioma; Malignant Neoplasm Of Lung; Benign Prostatic Hypertrophy; Carcinoma; Tumor Angiogenesis; Blepharoptosis; Nervousness; Lymphoma; Pathologic Neovascularization; Cell Invasion; Bladder Neoplasm|
|Related Pathways||Cell Motility; Cell Migration; Cell Proliferation; Smooth Muscle Contraction; Brain Development; Peristalsis; Menopause; Muscle Contraction; Neuron Development; Localization; Cytokinesis; Angiogenesis; Pathogenesis; Regulation Of Smooth Muscle Contraction|
|Background||Caldesmon is a potential actomyosin regulatory protein found in smooth muscle and nonmuscle cells. The predicted smooth-muscle polypeptide is 793 amino acids long. The high molecular weight caldesmon(h-CaD) is predominantly expressed in smooth muscles, whereas the low molecular weight caldesmon(l-CaD) is widely distributed in nonmuscle tissues and cells. Hayashi et al.(1992) demonstrated that the human CDM gene is composed of 14 exons.|
|Scientific References||PMID: 7876150 by Yamashiro S., et al. Characterization of the COOH terminus of non-muscle caldesmon mutants lacking mitosis-specific phosphorylation sites.|
PMID: 1986309 by Yamashiro S., et al. Phosphorylation of non-muscle caldesmon by p34cdc2 kinase during mitosis.
Our Boster Quality Guarantee for Anti-Caldesmon (Smooth) Cald1 Antibody (Monoclonal, hHCD) covers its use in the following applications.Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, mouse, rabbit, By Heat
Western blot, 0.25-0.5μg/ml, Human, mouse, rabbit
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
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