Anti-Caldesmon (Smooth) Cald1 Antibody (Monoclonal, hHCD)

Caldesmon/CALD1 antibody

Boster Bio Anti-Caldesmon (Smooth) Cald1 Antibody (Monoclonal, hHCD) catalog # MA1009. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rabbit.

Product Info Summary

SKU: MA1009
Size: 100μg/vial
Reactive Species: Human, Mouse, Rabbit
Host: Mouse
Application: IHC, WB

Product Name

Anti-Caldesmon (Smooth) Cald1 Antibody (Monoclonal, hHCD)

View all Caldesmon/CALD1 Antibodies

SKU/Catalog Number

MA1009

Size

100μg/vial

Form

Liquid

Description

Boster Bio Anti-Caldesmon (Smooth) Cald1 Antibody (Monoclonal, hHCD) catalog # MA1009. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rabbit.

Storage & Handling

At -20˚C for one year and at 4˚C for one month from date of receipt. Avoid repeated freezing and thawing.

Cite This Product

Anti-Caldesmon (Smooth) Cald1 Antibody (Monoclonal, hHCD) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1009)

Host

Mouse

Contents

Each vial contains 50% glycerol, 1.2% Sodium acetate, 1% BSA, 0.02% NaN3.

Clonality

Monoclonal

Clone Number

hHCD

Isotype

Mouse IgG1

Immunogen

Human uterus smooth muscle extract.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

MA1009 is reactive to Cald1 in Human, Mouse, Rabbit

Applications

MA1009 is guaranteed for IHC, WB Boster Guarantee

Background of Caldesmon/CALD1

Caldesmon is a potential actomyosin regulatory protein found in smooth muscle and nonmuscle cells. The predicted smooth-muscle polypeptide is 793 amino acids long. The high molecular weight caldesmon (h-CaD) is predominantly expressed in smooth muscles, whereas the low molecular weight caldesmon (l-CaD) is widely distributed in nonmuscle tissues and cells. Hayashi et al. (1992) demonstrated that the human CDM gene is composed of 14 exons.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, mouse, rabbit, By Heat
Western blot, 0.25-0.5μg/ml, Human, mouse, rabbit

Validation Images & Assay Conditions

Gene/Protein Information For Cald1 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q62736

Gene ID

25687

Gene Name

Cald1

Full Name

Non-muscle caldesmon

Weight

60.584kDa

Superfamily

caldesmon family

Alternative Names

CAD; CALD1; caldesmon 1; Caldesmon; CDM; CDMH-CAD; HCAD; LCAD; L-CAD; MGC21352; NAG22 Cald1|l-Cad|caldesmon 1|non-muscle caldesmon|CDM|L-caldesmon|h-caldesmon

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Cald1, check out the Cald1 Infographic

Cald1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Cald1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

No publications found for MA1009

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6 Customer Q&As for Anti-Caldesmon (Smooth) Cald1 Antibody (Monoclonal, hHCD)

Question

Would MA1009 anti-Caldesmon (Smooth) antibody (Monoclonal, hHCD) work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2020-03-16

Answer

It shows on the product datasheet, MA1009 anti-Caldesmon (Smooth) antibody (Monoclonal, hHCD) as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-03-16

Question

Is this MA1009 anti-Caldesmon (Smooth) antibody (Monoclonal, hHCD) reactive to the isotypes of CALD1?

Verified Customer

Verified customer

Asked: 2020-02-03

Answer

The immunogen of MA1009 anti-Caldesmon (Smooth) antibody (Monoclonal, hHCD) is Human uterus smooth muscle extract. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2020-02-03

Question

Would anti-Caldesmon (Smooth) antibody (Monoclonal, hHCD) MA1009 work on primate WB with skin?

Verified Customer

Verified customer

Asked: 2019-11-12

Answer

Our lab technicians have not tested anti-Caldesmon (Smooth) antibody (Monoclonal, hHCD) MA1009 on primate. You can run a BLAST between primate and the immunogen sequence of anti-Caldesmon (Smooth) antibody (Monoclonal, hHCD) MA1009 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate skin in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-11-12

Question

Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma using anti-Caldesmon (Smooth) antibody (Monoclonal, hHCD) MA1009. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-05-31

Answer

Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-05-31

Question

Here is the WB image, lot number and protocol we used for cervix carcinoma using anti-Caldesmon (Smooth) antibody (Monoclonal, hHCD) MA1009. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2017-06-16

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-06-16

Question

I see that the anti-Caldesmon (Smooth) antibody (Monoclonal, hHCD) MA1009 works with WB, what is the protocol used to produce the result images on the product page?

E. Parker

Verified customer

Asked: 2016-10-07

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2016-10-07

Size

Total: $315

SKU:MA1009

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MA1009
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$315.00

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