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- Table of Contents
CALM1,CALM2,CALM3 antibody (M06609) performs well in Flow Cytometry, IP, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 1 Publication
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Immunohistochemical analysis of paraffin-embedded human kidney, using Calmodulin Antibody (M06609)
CALM1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Calmodulin Antibody (M06609)overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
CALM1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Calmodulin Antibody (M06609)overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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Anti-Calmodulin Rabbit Monoclonal Antibody
CALM1,CALM2,CALM3 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | Flow Cytometry, IP, IF, IHC, ICC, WB |
Tested Applications
CALM1,CALM2,CALM3 antibody was positively detected by WB in:
human MCF-7 whole cell, human HepG2 whole cell, human Hela whole cell, human U20S whole cell, rat skeletal muscle tissue, rat RH35 whole cell, mouse skeletal muscle tissue, mouse NIH/3T3 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
CALM1,CALM2,CALM3 antibody was positively detected by IHC in:
human kidney tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
WB 1:1000-5000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:20
FC 1:20
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
ICC
IF
IP
FC
Human
Tested
Tested
Recommend
Recommend
Recommend
Recommend
Rat
Tested
Recommend
Recommend
Recommend
Recommend
Mouse
Tested
Recommend
Recommend
Recommend
Recommend
Product Information
Product Name
Anti-Calmodulin Rabbit Monoclonal Antibody
View all CALM1,CALM2,CALM3 Antibodies
View all CALM1,CALM2,CALM3 Antibodies
Form
Liquid
Uniprot
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
BFB-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Calmodulin
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
17 kDa
Cite This Product
Anti-Calmodulin Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M06609)
Protocols
Western Blot protocol for CALM1,CALM2,CALM3 antibody
Immunohistochemistry (IHC) protocol for CALM1,CALM2,CALM3 antibody
Publications
Application:
Species:
Effects of sodium fluoride on hyperactivation and Ca2+ signaling pathway in sperm from mice: an in vivo study
Published: 2010-01-06
Journal: ARCHIVES OF TOXICOLOGY
Application: WB
Impact Factor: 3.31
Species: Mouse
PMID: 20052459
Review
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Product Promise
We promise Anti-Calmodulin Rabbit Monoclonal Antibody performs excellently in relevant applications for Flow Cytometry, IP, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
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M06609
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 1, 2026