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Product Info Summary
| SKU: | A02170-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-Calpain 3/CAPN3 Antibody Picoband®
View all Calpain 3/CAPN3 Antibodies
SKU/Catalog Number
A02170-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Calpain 3/CAPN3 Antibody Picoband® catalog # A02170-2. Tested in WB, FCM, ELISA applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Calpain 3/CAPN3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02170-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human Calpain 3/CAPN3 recombinant protein (Position: I42-D764). Human CAPN3 shares 93.5% and 94.2% amino acid (aa) sequence identity with mouse and rat CAPN3, respectively.
Reactive Species
A02170-2 is reactive to Calpain 3/CAPN3 in Human, Mouse
Observed Molecular Weight
99 kDa
Calculated molecular weight
94.3 kDa
Background of Calpain 3/CAPN3
Calpain-3 is a protein that in humans is encoded by the CAPN3 gene. Calpain, a heterodimer consisting of a large and a small subunit, is a major intracellular protease, although its function has not been well established. This gene encodes a muscle-specific member of the calpain large subunit family that specifically binds to titin. Mutations in this gene are associated with limb-girdle muscular dystrophies type 2A. Alternate promoters and alternative splicing result in multiple transcript variants encoding different isoforms and some variants are ubiquitously expressed.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02170-2 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Recommend Dilution
| Application | Dilution | Species |
|---|---|---|
| Western blot | 0.25-0.5 μg/ml | Mouse |
| ELISA | 0.1-0.5 μg/ml | - |
Tested application
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 µg per lane
Validation Images & Assay Conditions
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Western blot analysis of Calpain 3/CAPN3 using anti-Calpain 3/CAPN3 antibody (A02170-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse thymus tissue lysates,
Lane 2: mouse C2C12 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Calpain 3/CAPN3 antigen affinity purified polyclonal antibody (Catalog # A02170-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Calpain 3/CAPN3 at approximately 99 kDa. The expected band size for Calpain 3/CAPN3 is at 99 kDa.
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Flow Cytometry analysis of THP-1 cells using anti-Calpain 3/CAPN3 antibody (A02170-2).
Overlay histogram showing THP-1 cells stained with A02170-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Calpain 3/CAPN3 Antibody (A02170-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Specific Publications For Anti-Calpain 3/CAPN3 Antibody Picoband® (A02170-2)
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