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Product Info Summary
| SKU: | PA1520 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-CaMKK/CAMKK1 Antibody Picoband®
SKU/Catalog Number
PA1520
BA3630 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CaMKK/CAMKK1 Antibody catalog # PA1520. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CaMKK/CAMKK1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1520)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of mouse CaMKK, different from the related human sequence by three amino acids, and from the related rat sequence by two amino acids.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1520 is reactive to Camkk1 in Human, Mouse, Rat
Observed Molecular Weight
56 kDa
Calculated molecular weight
55.8 kDa
Background of Camkk1
CAMKK1, Calcium/calmodulin-dependent protein kinase kinase 1 is an enzyme that in humans is encoded by the CAMKK1 gene. The CAMKK1 gene is mapped to chromosome 17. The product of this gene belongs to the Serine/Threonine protein kinase family, and to the Ca (2+)/calmodulin-dependent protein kinase subfamily. This protein plays a role in the calcium/calmodulin-dependent (CaM) kinase cascade. Three transcript variants encoding two distinct isoforms have been identified for this gene.Camkk1binds calmodulin and activated Camk4 with a 6-fold increase in total activity and a 100-fold increase in Camk4 Ca (2+)-independent activity. Camkk1 catalyzed a 10-fold increase in the total activity of Camk1 and had no effect on Camk2. Cotransfection of COS-7 cells with Camkk1 and Camk4 resulted in a 14-fold increase in CRE-binding protein-dependent transcription compared with Camk4 alone, suggesting that Camkk1 enhances Camk4-mediated transcriptional regulation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1520 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Rat, Mouse
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: human Jurkat whole cell, human Hela whole cell, human K562 whole cell, human U87 whole cell, rat brain tissue, mouse brain tissue
IHC: Rat Brain Tissue
Validation Images & Assay Conditions
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Western blot analysis of CAMKK1 using anti-CAMKK1 antibody (PA1520).
Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human K562 whole cell lysates,
Lane 4: human U87 whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CAMKK1 antigen affinity purified polyclonal antibody (PA1520) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for CAMKK1 at approximately 56 kDa. The expected band size for CAMKK1 is at 56 kDa.
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Anti-CaMKK antibody, PA1520, IHC(P)
IHC(P): Rat Brain Tissue
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IHC analysis of CAMKK1 using anti-CAMKK1 antibody (PA1520).
CAMKK1 was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CAMKK1 Antibody (PA1520) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Specific Publications For Anti-CaMKK/CAMKK1 Antibody Picoband® (PA1520)
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1 Customer Q&As for Anti-CaMKK/CAMKK1 Antibody Picoband®
Question
We are currently using anti-CaMKK/CAMKK1 antibody PA1520 for rat tissue, and we are satisfied with the IHC-P results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-09
Answer
The anti-CaMKK/CAMKK1 antibody (PA1520) has not been tested for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-09
