Anti-Carabin TBC1D10C Antibody

Product Name

Anti-Carabin TBC1D10C Antibody

View all TBC1D10C Antibodies

SKU/Catalog Number

A11412

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Carabin TBC1D10C Antibody (Catalog # A11412). Tested in ELISA, WB, IHC-P applications. This antibody reacts with Human, Mouse.

Storage & Handling

Carabin antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-Carabin TBC1D10C Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11412)

Host

Rabbit

Contents

Carabin Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Carabin antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human Carabin. The immunogen is located within the last 50 amino acids of Carabin.

Reactive Species

A11412 is reactive to TBC1D10C in Human, Mouse

Observed Molecular Weight

68 kDa

Calculated molecular weight

49.7 kDa

Background of TBC1D10C

Antigen binding by the T-cell receptor (TCR) is one of the critical first steps in the immune response, triggering a cascade of signaling pathways that ultimately lead to T-cell activation. Screening a yeast two-hybrid screen of a human T-cell cDNA library with calcineurin, a protein phosphatase involved in multiple signaling pathways including T-cell activation, resulted in the identification of Carabin, a member of the TBC1 domain family of proteins, as a calcineurin-binding protein. Unlike other members of the TBC1 domain protein family which are thought to have a role in regulating cell growth and differentiation, further experiments demonstrated that Carabin is part of a negative regulatory loop for the intracellular TCR signaling pathway as well as an inhibitor of the Ras signaling pathway, suggesting that Carabin may also mediate crosstalk between calcineurin and Ras. Carabin antibody does not recognize TBC1D10A or TBC1D10B. Carabin is known to exist in multiple isoforms.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A11412 is guaranteed for ELISA, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Carabin antibody can be used for detection of Carabin by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information TBC1D10C

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