Anti-Cardiac Troponin T/TNNT2 Antibody Picoband™

Product Name

Anti-Cardiac Troponin T/TNNT2 Antibody Picoband™

View all TNNT2 Antibodies

SKU/Catalog Number

A01154-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Cardiac Troponin T/TNNT2 Antibody Picoband™ catalog # A01154-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Cardiac Troponin T/TNNT2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01154-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na₂HPO₄, 0.05mg NaN₃.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived human Cardiac Troponin T recombinant protein (Position: K88-K298).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A01154-1 is reactive to TNNT2 in Human, Mouse, Rat

Applications

A01154-1 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

43 kDa

Calculated molecular weight

35.924kDa

Background of TNNT2

Cardiac muscle troponin T (cTnT), is a protein which in humans is encoded by the TNNT2 gene. It is mapped to 1q32.1. The protein encoded by this gene is the tropomyosin-binding subunit of the troponin complex, which is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentration. Mutations in this gene have been associated with familial hypertrophic cardiomyopathy as well as with dilated cardiomyopathy. Transcripts for this gene undergo alternative splicing that results in many tissue-specific isoforms, however, the full-length nature of some of these variants has not yet been determined.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

Submit A Review

Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Direct ELISA, 0.1-0.5μg/ml

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of Cardiac Troponin T using anti-Cardiac Troponin T antibody (A01154-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat heart tissue lysates,
Lane 2: rat heart tissue lysates,
Lane 3: mouse heart tissue lysates,
Lane 4: mouse heart tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cardiac Troponin T antigen affinity purified polyclonal antibody (Catalog # A01154-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cardiac Troponin T at approximately 43KD. The expected band size for Cardiac Troponin T is at 36KD.

Gene/Protein Information For TNNT2 (Source: Uniprot.org, NCBI)

Alternative Names

Cardiac Muscle Troponin T; Cardiomyopathy, Dilated 1D (Autosomal Dominant); Cardiomyopathy, Hypertrophic; CMD1D; CMH2; CMPD2; cTnT; LVNC6; RCM3; TNNT2; TnTC; troponin T type 2 (cardiac); Troponin T; troponin T, cardiac muscle; Troponin T2, Cardiac Type; Truncated Cardiac Troponin T TNNT2 CMD1D, CMH2, CMPD2, LVNC6, RCM3, TnTC, cTnT troponin T2, cardiac type troponin T, cardiac muscle|cardiomyopathy, dilated 1D (autosomal dominant)|cardiomyopathy, hypertrophic 2|troponin T type 2 (cardiac)|truncated cardiac troponin T

For more info on TNNT2, check out the TNNT2 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for TNNT2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Take me to the TNNT2 Infographic

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis