Click image to see more details
Product Info Summary
| SKU: | M01154 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Troponin T TNNT2 Rabbit Monoclonal Antibody
SKU/Catalog Number
M01154
BM4509 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Troponin T TNNT2 Rabbit Monoclonal Antibody catalog # M01154. Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Troponin T TNNT2 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01154)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
FDC-20
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Troponin T
Reactive Species
M01154 is reactive to TNNT2 in Human, Mouse, Rat
Observed Molecular Weight
40 kDa
Calculated molecular weight
35.9 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01154 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-5000
IHC 1:50-200
Positive Control
WB: rat heart tissue, mouse heart whole cell
IHC: human heart muscle tissue
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of Troponin T using anti-Troponin T antibody (M01154).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat heart tissue lysates,
Lane 2: mouse heart whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Troponin T antigen affinity purified monoclonal antibody (M01154) at 1:5000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Troponin T at approximately 40 kDa. The expected band size for Troponin T is at 36 kDa.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded human heart muscle, using Troponin T Antibody.
Specific Publications For Anti-Troponin T TNNT2 Rabbit Monoclonal Antibody (M01154)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Troponin T TNNT2 Rabbit Monoclonal Antibody?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-Troponin T TNNT2 Rabbit Monoclonal Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
5 Customer Q&As for Anti-Troponin T TNNT2 Rabbit Monoclonal Antibody
Question
Could you please recommend some antibodies against human cardiac troponin T? I'm trying to find an antibody pair for my immunoassay.
Verified customer
Asked: 2021-12-07
Answer
We can recommend M01154 and M01154-1.
Boster Scientific Support
Answered: 2022-01-08
Question
Is a blocking peptide available for product anti-Troponin T Rabbit Monoclonal antibody (M01154)?
Verified Customer
Verified customer
Asked: 2020-02-13
Answer
We do provide the blocking peptide for product anti-Troponin T Rabbit Monoclonal antibody (M01154). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2020-02-13
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cardiac ventricle using anti-Troponin T Rabbit Monoclonal antibody M01154. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-10-18
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-18
Question
Is this M01154 anti-Troponin T Rabbit Monoclonal antibody reactive to the isotypes of TNNT2?
E. Anderson
Verified customer
Asked: 2019-07-04
Answer
The immunogen of M01154 anti-Troponin T Rabbit Monoclonal antibody is A synthesized peptide derived from human Troponin T. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-07-04
Question
I was wanting to use to test anti-Troponin T Rabbit Monoclonal antibody M01154 on rat cardiac ventricle for research purposes, then I may be interested in using anti-Troponin T Rabbit Monoclonal antibody M01154 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-08-04
Answer
The products we sell, including anti-Troponin T Rabbit Monoclonal antibody M01154, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-08-04
