Anti-CARKL SHPK Antibody

CARKL antibody

Boster Bio Anti-CARKL SHPK Antibody (Catalog # A09991). Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A09991
Size: 100 µl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IHC, WB

Product Name

Anti-CARKL SHPK Antibody

View all CARKL Antibodies

SKU/Catalog Number

A09991

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-CARKL SHPK Antibody (Catalog # A09991). Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CARKL SHPK Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09991)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from N-terminal of human CARKL.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A09991 is reactive to SHPK in Human, Mouse, Rat

Applications

A09991 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

51.491kDa

Background of CARKL

Acts as a modulator of macrophage activation through control of glucose metabolism By similarity.

Touchman J.W., Genome Res. 10:165-173(2000).<

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100

Validation Images & Assay Conditions

Gene/Protein Information For SHPK (Source: Uniprot.org, NCBI)

Gene Name

SHPK

Full Name

Sedoheptulokinase

Weight

51.491kDa

Superfamily

FGGY kinase family

Alternative Names

Carbohydrate kinase-like protein; carbohydrate kinase-like; CARKLEC 2.7.1.14; sedoheptulokinase; SHKFLJ32478 SHPK CARKL, SHK sedoheptulokinase sedoheptulokinase|carbohydrate kinase-like protein

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on SHPK, check out the SHPK Infographic

SHPK infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for SHPK: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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4 Customer Q&As for Anti-CARKL SHPK Antibody

Question

We are currently using anti-CARKL antibody A09991 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on primate tissues as well?

Verified Customer

Verified customer

Asked: 2019-05-17

Answer

The anti-CARKL antibody (A09991) has not been validated for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-05-17

Question

I would like to test anti-CARKL antibody A09991 on human brain for research purposes, then I may be interested in using anti-CARKL antibody A09991 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2018-08-21

Answer

The products we sell, including anti-CARKL antibody A09991, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2018-08-21

Question

Will anti-CARKL antibody A09991 work for IHC with brain?

P. Bhatt

Verified customer

Asked: 2015-07-17

Answer

According to the expression profile of brain, SHPK is highly expressed in brain. So, it is likely that anti-CARKL antibody A09991 will work for IHC with brain.

Boster Scientific Support

Answered: 2015-07-17

Question

Would A09991 anti-CARKL antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

D. Li

Verified customer

Asked: 2014-12-22

Answer

It shows on the product datasheet, A09991 anti-CARKL antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2014-12-22

Order DetailsPrice
A09991-100uL

100uL

$360

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A09991
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