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Product Info Summary
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Anti-Cas5 CASP5 Antibody
Boster Bio Anti-Cas5 CASP5 Antibody catalog # A05259. Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cas5 CASP5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05259)
0.01 M Sodium Phosphate
0.25 M Sodium Chloride
0.02% (w/v) Sodium Azide
Anti-Caspase-5 antibody was prepared from whole rabbit serum produced by repeated immunizations with a 14 amino acid synthetic peptide from the internal of human Caspase-5.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
No cross reactivity with other proteins.
A05259 is reactive to CASP5 in Human
A05259 is guaranteed for WB Boster Guarantee
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Western Blot, 1:500- 1:2, 000, human, mouse, rat, chimpanzee, bovine, rat
Direct ELISA, 1:10, 000 - 1:50, 000, human, mouse, rat, chimpanzee, bovine, rat
Validation Images & Assay Conditions
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Western blot analysis of Cas5 expression in Lane 1 contains a lysate from AS cells transfected with empty vector. Lane 2 contains lysate from AS cells transfected with hCas5 (predicted MW 125 kDa). Lane 3 contains lysate from BE2 cells that have been shown to contain a high level of hCas mRNA (as tested by Northern blot). Lane 4 contains lysate from human muscle tissue that also shows high levels of hCas mRNA. Cas5 at 125KD was detected using rabbit anti-Cas5 Antigen Affinity purified polyclonal antibody (Catalog # A05259). Personal Communication. Carol Thiele, NCI, Bethesda, MD.
Gene/Protein Information For CASP5 (Source: Uniprot.org, NCBI)
peptidase C14A family
CASP5; CASP-5; caspase 5, apoptosis-related cysteine peptidase; caspase 5, apoptosis-related cysteine protease; Caspase5; Caspase-5; EC 3.4.22; EC 188.8.131.52; ICE(rel)III; ICE(rel)-III; ICEREL-III; ICH3; ICH-3; MGC141966; Protease ICH-3; Protease TY; TY protease CASP5 ICE(rel)III, ICEREL-III, ICH-3 caspase 5 caspase-5|TY protease|caspase 5 variant X1|caspase 5, apoptosis-related cysteine peptidase|caspase 5, apoptosis-related cysteine protease|protease ICH-3|protease TY*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".
For more info on CASP5, check out the CASP5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CASP5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected]
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