Product Info Summary
| SKU: | A02468 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, WB |
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Product info
Product Name
Anti-CASK Antibody
SKU/Catalog Number
A02468
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-CASK Antibody (Catalog # A02468). Tested in ELISA, WB, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
CASK antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-CASK Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02468)
Host
Rabbit
Contents
CASK Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
CASK antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human CASK. The immunogen is located within amino acids 760 - 810 of CASK.
Cross-reactivity
At least six alternatively spliced transcript variants have been observed.
Reactive Species
A02468 is reactive to CASK in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
105.1 kDa
Background of CASK
CASK (Calcium/calmodulin-dependent serine protein kinase), a conserved multi-domain scaffolding protein, belongs to a MAGUK (membrane-associated guanylate kinase homologs) subfamily and is involved in cell junction organization, tumor suppression and signaling. It is characterized by a novel domain structure that consists of a calcium/calmodulin-dependent protein kinase domain followed by PDZ, SH3 and guanylate kinase-like (GUK) domains. CASK is ubiquitously expressed and significantly greater in brain where it is thought to be involved in signaling at neuronal synapses. CASK interacts with CASKINs and defects in CASK are the cause of mental retardation X-linked CASK-related.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02468 is guaranteed for ELISA, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
CASK antibody can be used for detection of CASK by Western blot at 1 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of CASK in mouse brain tissue lysate with CASK antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
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Immunofluorescence of CASK in human brain tissue with CASK antibody at 20 μg/mL.
Specific Publications For Anti-CASK Antibody (A02468)
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