This website uses cookies to ensure you get the best experience on our website.
- Table of Contents
CASP14 antibody (PA2104) performs well in IHC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 1 Publication
Filter:
Anti-Caspase-14 antibody, PA2104, IHC(P)
IHC(P): Human Tonsil Tissue
IHC(P): Human Tonsil Tissue
Bundle Save
Anti-Caspase-14/CASP14 Antibody Picoband®
CASP14 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | IHC, WB |
Tested Applications
CASP14 antibody was positively detected by WB in:
Rat Brain Tissue, Rat Liver Tissue, Rat Spleen Tissue, A431 Whole Cell, NIH3T3 Whole Cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
CASP14 antibody was positively detected by IHC in:
Human Tonsil Tissue, Human Oesophagus Squama Cancer Tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Human
Western blot
0.1-0.5μg/ml
Human, Mouse, Rat
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
Filter:
Boster Guarantee:What is this?
WB
IHC
IF
Rat
Tested
Human
Tested
Recommend
Rabbit
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Monkey
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-Caspase-14/CASP14 Antibody Picoband®
View all CASP14 Antibodies
View all CASP14 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Caspase-14, different from the related rat and mouse sequences by four amino acids.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
28 kDa
Calculated Molecular Weight
27.7 kDa
Background of CASP14
Caspase 14 is an enzyme that in humans is encoded by the CASP14 gene. CASP14 belongs the evolutionarily conserved caspase family. CASP14 was assigned to chromosome 19p13.1. Using Northern blot and semiquantitative RT-PCR analyses, it was showed that CASP14 was transcriptionally upregulated during maintenance of confluent cultures of human epidermal keratinocytes. The expression and processing of this caspase may be involved in keratinocyte terminal differentiation, which is important for the formation of the skin barrier. Caspase 14 seems to play a role in keratinocyte differentiation and cornification and probably regulates maturation of the epidermis by proteolytically processing filaggrin.
Cite This Product
Anti-Caspase-14/CASP14 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2104)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Rabbit
95%
High Homology
Dog
90%
High Homology
Monkey
90%
High Homology
Rat
85%
Moderate Homology
Mouse
80%
Moderate Homology
Cow
80%
Moderate Homology
Sheep
80%
Moderate Homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for CASP14 antibody
Immunohistochemistry (IHC) protocol for CASP14 antibody
Publications
Application:
Epigallocatechin-3-gallate (EGCG) inhibits imiquimod-induced psoriasis-like inflammation of BALB/c mice
PMID: 27581210
Review
Have you used Anti-Caspase-14/CASP14 Antibody Picoband®?
Submit a review and receive $370 in product credits, a $185 Amazon gift card, or our carefully curated gift bundles.
Product Promise
We promise Anti-Caspase-14/CASP14 Antibody Picoband® performs excellently in relevant applications for IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
PA2104
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 5, 2026