Anti-Caspase-14/CASP14 Antibody

Boster Bio Anti-Caspase-14/CASP14 Antibody catalog # PA2104. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 1 publication(s).

Product Info Summary

SKU: PA2104
Size: 100μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IHC, WB

Product Name

Anti-Caspase-14/CASP14 Antibody

See all Caspase-14 products

SKU/Catalog Number

PA2104

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Caspase-14/CASP14 Antibody catalog # PA2104. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Caspase-14/CASP14 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2104)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Caspase-14 (223-242aa KARKTNPEIQSTLRKRLYLQ), different from the related rat and mouse sequences by four amino acids.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

PA2104 is reactive to CASP14 in Human, Mouse, Rat

Applications

PA2104 is guaranteed for IHC, WB Boster Guarantee

*Blocking peptide can be purchased at $150. Contact us for more information.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat

Validation Images & Assay Conditions

Gene/Protein Information For CASP14 (Source: Uniprot.Org, NCBI)

Uniprot ID

P31944

Gene ID

23581

Gene Name

CASP14

Full Name

Caspase-14

Weight

27.68kDa

Superfamily

peptidase C14A family

Alternative Names

apoptosis-related cysteine protease; CASP14; CASP-14; caspase 14, apoptosis-related cysteine peptidase; caspase 14, apoptosis-related cysteine protease; Caspase14; Caspase-14; EC 3.4.22.-; MGC119078; MGC119079; MICE CASP14 ARCI12 caspase 14 caspase-14|CASP-14|apoptosis-related cysteine protease|caspase 14, apoptosis-related cysteine peptidase|caspase 14, apoptosis-related cysteine protease

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CASP14, check out the CASP14 Infographic

CASP14 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CASP14: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

PA2104 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Epigallocatechin-3-gallate (EGCG) inhibits imiquimod-induced psoriasis-like inflammation of BALB/c mice

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7 Customer Q&As for Anti-Caspase-14/CASP14 Antibody

Question

We are currently using anti-Caspase-14/CASP14 antibody PA2104 for human tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on primate tissues as well?

Verified Customer

Verified customer

Asked: 2020-03-17

Answer

The anti-Caspase-14/CASP14 antibody (PA2104) has not been validated for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-03-17

Question

I see that the anti-Caspase-14/CASP14 antibody PA2104 works with IHC, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2020-01-08

Answer

You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2020-01-08

Question

I have attached the WB image, lot number and protocol we used for keratinocyte using anti-Caspase-14/CASP14 antibody PA2104. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-11-13

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-11-13

Question

I have a question about product PA2104, anti-Caspase-14/CASP14 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-09-19

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA2104 anti-Caspase-14/CASP14 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-09-19

Question

Is a blocking peptide available for product anti-Caspase-14/CASP14 antibody (PA2104)?

A. Brown

Verified customer

Asked: 2017-11-24

Answer

We do provide the blocking peptide for product anti-Caspase-14/CASP14 antibody (PA2104). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2017-11-24

Question

I was wanting to use your anti-Caspase-14/CASP14 antibody for IHC for mouse keratinocyte on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse keratinocyte identification?

B. Jones

Verified customer

Asked: 2014-12-12

Answer

As indicated on the product datasheet, PA2104 anti-Caspase-14/CASP14 antibody has been tested for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse keratinocyte in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2014-12-12

Question

Would anti-Caspase-14/CASP14 antibody PA2104 work on feline WB with brain?

R. Krishna

Verified customer

Asked: 2013-04-17

Answer

Our lab technicians have not tested anti-Caspase-14/CASP14 antibody PA2104 on feline. You can run a BLAST between feline and the immunogen sequence of anti-Caspase-14/CASP14 antibody PA2104 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline brain in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2013-04-17

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PA2104
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