Anti-Caspase-14/CASP14 Antibody

Product Name

Anti-Caspase-14/CASP14 Antibody

View all Caspase-14 Antibodies

SKU/Catalog Number

PA2104

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Caspase-14/CASP14 Antibody catalog # PA2104. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Caspase-14/CASP14 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2104)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Caspase-14, different from the related rat and mouse sequences by four amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA2104 is reactive to Casp14 in Human, Mouse, Rat

Applications

PA2104 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

28 kDa

Calculated molecular weight

29.458kDa

Background of Caspase-14

Caspase 14 is an enzyme that in humans is encoded by the CASP14 gene. CASP14 belongs the evolutionarily conserved caspase family. CASP14 was assigned to chromosome 19p13.1. Using Northern blot and semiquantitative RT-PCR analyses, it was showed that CASP14 was transcriptionally upregulated during maintenance of confluent cultures of human epidermal keratinocytes. The expression and processing of this caspase may be involved in keratinocyte terminal differentiation, which is important for the formation of the skin barrier. Caspase 14 seems to play a role in keratinocyte differentiation and cornification and probably regulates maturation of the epidermis by proteolytically processing filaggrin.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat

Validation Images & Assay Conditions

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Anti-Caspase-14 antibody, PA2104, Western blotting
All lanes: Anti Caspase-14 (PA2104) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Rat Liver Tissue Lysate at 50ug
Lane 3: Rat Spleen Tissue Lysate at 50ug
Lane 4: A431 Whole Cell Lysate at 40ug
Lane 5: NIH3T3 Whole Cell Lysate at 40ug
Predicted bind size: 28KD
Observed bind size: 28KD

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Anti-Caspase-14 antibody, PA2104, IHC(P)
IHC(P): Human Tonsil Tissue

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Anti-Caspase-14 antibody, PA2104, IHC(P)
IHC(P): Human Oesophagus Squama Cancer Tissue

Gene/Protein Information For Casp14 (Source: Uniprot.org, NCBI)

Alternative Names

apoptosis-related cysteine protease; CASP14; CASP-14; caspase 14, apoptosis-related cysteine peptidase; caspase 14, apoptosis-related cysteine protease; Caspase14; Caspase-14; EC 3.4.22.-; MGC119078; MGC119079; MICE Casp14|MIC, MICE, mini, mini-ICE|caspase 14|caspase-14|CASP-14

For more info on Casp14, check out the Casp14 Infographic

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In this infographic, you will see the following information for Casp14: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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