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Product Info Summary
|Reactive Species:||Human, Mouse, Rat|
|Application:||IP, IF, WB|
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Anti-Caspase-14 Rabbit Monoclonal Antibody
Boster Bio Anti-Caspase-14 Rabbit Monoclonal Antibody catalog # M05143. Tested in IP, IF, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Caspase-14 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05143)
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
A synthesized peptide derived from human Caspase-14
M05143 is reactive to CASP14 in Human, Mouse, Rat
M05143 is guaranteed for IP, IF, WB Boster Guarantee
*Blocking peptide can be purchased at $150. Contact us for more information.
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Flow Cytometry 1:50
Validation Images & Assay Conditions
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Western blot analysis of Caspase-14 expression in Human skin lysate (M05143).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CASP14 monoclonal antibody (Catalog # M05143) overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CASP14
Gene/Protein Information For CASP14 (Source: Uniprot.Org, NCBI)
peptidase C14A family
apoptosis-related cysteine protease; CASP14; CASP-14; caspase 14, apoptosis-related cysteine peptidase; caspase 14, apoptosis-related cysteine protease; Caspase14; Caspase-14; EC 3.4.22.-; MGC119078; MGC119079; MICE CASP14 ARCI12 caspase 14 caspase-14|CASP-14|apoptosis-related cysteine protease|caspase 14, apoptosis-related cysteine peptidase|caspase 14, apoptosis-related cysteine protease*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on CASP14, check out the CASP14 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CASP14: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
No publications found for M05143
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