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Product Info Summary
| SKU: | M08617 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Caspr Monoclonal Antibody
SKU/Catalog Number
M08617
BM5276 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Caspr Monoclonal Antibody catalog # M08617. Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Caspr Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M08617)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ACEI-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Caspr Contactin-associated protein 2 (Caspr2) is a type I transmembrane protein and member of the neurexin superfamily that mediates nervous system cell-cell interactions through the Neurexin IV-Caspr-Paranodin (NCP) complex.
Reactive Species
M08617 is reactive to CNTNAP1 in Human, Mouse, Rat
Observed Molecular Weight
165 kDa
Calculated molecular weight
156.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M08617 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
Positive Control
WB: U87-MG cell
Validation Images & Assay Conditions
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Western blot analysis of Caspr expression in U87-MG cell lysate.
Specific Publications For Anti-Caspr Monoclonal Antibody (M08617)
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Customer Q&As
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7 Customer Q&As for Anti-Caspr Monoclonal Antibody
Question
My question regarding product M08617, anti-Caspr Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-02-12
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M08617 anti-Caspr Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-02-12
Question
Is a blocking peptide available for product anti-Caspr Monoclonal antibody (M08617)?
Verified Customer
Verified customer
Asked: 2018-05-17
Answer
We do provide the blocking peptide for product anti-Caspr Monoclonal antibody (M08617). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2018-05-17
Question
See below the WB image, lot number and protocol we used for frontal cortex using anti-Caspr Monoclonal antibody M08617. Please let me know if you require anything else.
A. Zhang
Verified customer
Asked: 2015-03-23
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-03-23
Question
I am interested in to test anti-Caspr Monoclonal antibody M08617 on rat frontal cortex for research purposes, then I may be interested in using anti-Caspr Monoclonal antibody M08617 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
O. Zhao
Verified customer
Asked: 2014-07-28
Answer
The products we sell, including anti-Caspr Monoclonal antibody M08617, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2014-07-28
Question
We are currently using anti-Caspr Monoclonal antibody M08617 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on bovine tissues as well?
D. Evans
Verified customer
Asked: 2013-06-24
Answer
The anti-Caspr Monoclonal antibody (M08617) has not been validated for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-06-24
Question
Does anti-Caspr Monoclonal antibody M08617 work on primate WB with frontal cortex?
K. Carter
Verified customer
Asked: 2013-02-08
Answer
Our lab technicians have not validated anti-Caspr Monoclonal antibody M08617 on primate. You can run a BLAST between primate and the immunogen sequence of anti-Caspr Monoclonal antibody M08617 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate frontal cortex in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-02-08
Question
Is this M08617 anti-Caspr Monoclonal antibody reactive to the isotypes of CNTNAP1?
D. Johnson
Verified customer
Asked: 2013-01-02
Answer
The immunogen of M08617 anti-Caspr Monoclonal antibody is A synthesized peptide derived from human Caspr Contactin-associated protein 2 (Caspr2) is a type I transmembrane protein and member of the neurexin superfamily that mediates nervous system cell-cell interactions through the Neurexin IV-Caspr-Paranodin (NCP) complex. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2013-01-02
