|Validated Species:||Human, Mouse, Rat|
Data & Images
|Product Name||Anti-Cathepsin L Antibody (Monoclonal, 33/2)|
|Description||Mouse IgG monoclonal antibody for Cathepsin L, cathepsin L1 (CTSL1) detection. Tested with WB, IHC-F in Human;mouse;rat. No cross reactivity with other proteins.|
|Cite This Product||Anti-Cathepsin L Antibody (Monoclonal, 33/2) 33/2 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1014)|
|Replacement Item||This antibody may replace the following items: sc-31836|sc-7582|sc-30107|sc-74399|sc-31837|sc-7581|sc-74497|sc-31838|sc-31840 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(F).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||Procathepsin L isolated from the human lung cancer cell line EPLC 32M1.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
*carrier free antibody available upon request.
|Concentration||Add 1ml of PBS buffer will yield a concentration of 100ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Cathepsin L1|
|Molecular Weight||37660 MW|
|Protein Function||Important for the overall degradation of proteins in lysosomes. Procathepsin L is required for maximal stimulation of steroidogenesis by TIMP1. .|
|Tissue Specificity||Both mature cathepsin L1 and procathepsin L are found in the upper epidermis. The lower epidermis predominantly contains procathepsin L. In seminiferous tubules expression is greater at stages VI-VII than at stages IX-XII. .|
|Sequence Similarities||Belongs to the peptidase C1 family.|
|Subcellular Localization||Lysosome .|
|Alternative Names||Cathepsin L1;18.104.22.168;Cathepsin L;Cyclic protein 2;CP-2;Major excreted protein;MEP;Procathepsin L;Cathepsin L1 heavy chain;Cathepsin L1 light chain;Ctsl;Ctsl1;|
|Research Areas|||neuroscience|cell adhesion proteins|membrane proteins| signal transduction|cytoskeleton / ecm|extracellular matrix|ecm enzymes|other enzymes||
Background for Cathepsin L1
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-Cathepsin L Antibody (Monoclonal, 33/2) by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Frozen Section), 4μg/ml, Human, mouse, rat, -|
Western blot, 2μg/ml, Human, mouse, rat
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.