|Reactivity||Human, Mouse, Rat|
|Ig Isotype||Mouse IgG1|
|Product Name||Anti-Cathepsin L Antibody (Monoclonal, 33/2)|
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Mouse IgG monoclonal antibody for Cathepsin L, cathepsin L1 (CTSL1) detection. Tested with WB, IHC-F in Human;mouse;rat. No cross reactivity with other proteins.|
|Cite This Product||Anti-Cathepsin L Antibody (Monoclonal, 33/2) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1014)|
|Contents/Buffer||Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.|
|Immunogen||Procathepsin L isolated from the human lung cancer cell line EPLC 32M1.|
|Reactivity||Human, Mouse, Rat|
Assay Dilutions Overview
Immunohistochemistry(Frozen Section), 4μg/ml, Human, mouse, rat, -
Western blot, 2μg/ml, Human, mouse, rat
Boster's Secondary Antibodies And IHC, WB Kits
The following reagents are used to generate the images below.Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(F).
Images And Assay Conditions
Boster Kit Box
Protein Target Info (Source: Uniprot.org)
|Protein Name||Cathepsin L1|
|Tissue Specificity||Both mature cathepsin L1 and procathepsin L are found in the upper epidermis. The lower epidermis predominantly contains procathepsin L. In seminiferous tubules expression is greater at stages VI-VII than at stages IX-XII. .|
|Alternative Names||Cathepsin L1;184.108.40.206;Cathepsin L;Cyclic protein 2;CP-2;Major excreted protein;MEP;Procathepsin L;Cathepsin L1 heavy chain;Cathepsin L1 light chain;Ctsl;Ctsl1;|
|Subcellular Localization||Lysosome .|
|Molecular Weight||37660 MW|
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Protein Function||Important for the overall degradation of proteins in lysosomes. Procathepsin L is required for maximal stimulation of steroidogenesis by TIMP1. .|
|Research Areas||Cell Adhesion Proteins, Cytoskeleton / Ecm, Ecm Enzymes, Extracellular Matrix, Membrane Proteins, Neuroscience, Signal Transduction
*You can search these to find other products in these research areas.
|Background||Cathepsin L is a lysosomal cysteine proteinase with a major role in intracellular protein catabolism. It also shows the most potent collagenolytic and elastinolytic activity in vitro of any of the cathepsins. Cathepsin L has been implicated in pathologic processes including myofibril necrosis in myopathies and in myocardial ischemia, and in the renal tubular response to proteinuria. Human liver cathepsin L consists of a heavy chain of about 25 kD and a light chain of about 5 kD. The gene is mapped to 9q21-q22. Cathepsin L is required for endothelial progenitor cell-induced neovascularization.|
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