Anti-Cathepsin L Ctsl Antibody (Monoclonal, 33/2)
Mouse IgG monoclonal antibody for Cathepsin L, cathepsin L1 (CTSL1) detection. Tested with WB, IHC-F in Human;mouse;rat. No cross reactivity with other proteins.
Anti-Cathepsin L Ctsl Antibody (Monoclonal, 33/2) Info At A Glance
|Reactivity:||Human, Mouse, Rat|
TPM1 is a member of the tropomyosin family which consists of a number of extremely conserved, extensively distributed 35-45 kDa actin-binding proteins that are involved in the contractile system of striated and smooth muscles and the cytoskeleton of non-muscle cells. Tropomyosin-1 is composed of 2 alpha-helical chains arranged as a coiled-coil. TPM1 is polymerized end to end alongside the two grooves of actin filaments and provides stability to the filaments. TPM1 binds to actin filaments in muscle and non-muscle cells. TPM1 also functions in association with the troponin complex to regulate the calcium-dependent interaction of actin and myosin during muscle contraction. In non-muscle cells TPM1 is implicated in stabilizing cytoskeleton actin filaments. Smooth muscle contraction is controlled by interaction with caldesmon.
Alternatively spliced transcript variants encoding a range of isoforms have been described in smooth muscle and non-muscle cells. TPM1 Isoform 1 is expressed in adult and fetal skeletal muscle and cardiac tissues, with higher expression levels in the cardiac tissues, whereas Isoform 10 is expressed in adult and fetal cardiac tissues, but not in skeletal muscle.
Mutations in the TPM1 gene are linked to type 3 familial hypertrophic cardiomyopathy.
|Product Name||Anti-Cathepsin L Ctsl Antibody (Monoclonal, 33/2)
See all Cathepsin L primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Monoclonal clone# G2 antibody for CTSL1 detection. Host: Mouse.Size: 100μg/vial. Tested applications: IHC-F. Reactive species: Human. CTSL1 information: Molecular Weight: 37660 MW; Subcellular Localization: Lysosome ; Tissue Specificity: Both mature cathepsin L1 and procathepsin L are found in the upper epidermis. The lower epidermis predominantly contains procathepsin L. In seminiferous tubules expression is greater at stages VI-VII than at stages IX-XII.|
|Cite This Product||Anti-Cathepsin L Ctsl Antibody (Monoclonal, 33/2) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1014)|
|Similar Products From
|Anti-Cathepsin L Ctsl Antibody (Monoclonal, 33/2) may replace the following items: sc 31836|sc 7582|sc 30107|sc 74399|sc 31837|sc 7581|sc 74497|sc 31838|sc 31840.|
|Reactivity/Species||Human, Mouse, Rat|
*Our Boster Guarantee covers the use of this product in the above tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products.
|Related Products||Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(F).
*Blocking peptide can be purchased at $50. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round.
|Immunogen||Procathepsin L isolated from the human lung cancer cell line EPLC 32M1.|
|Cross Reactivity||No cross reactivity with other proteins|
Gene/Protein Basic Information For Ctsl (Source: Uniprot.org, NCBI)
|NCBI Gene Id||25697|
|Species Of This Entry||Rat|
|Protein Name||Cathepsin L1|
|Superfamily||peptidase C1 family|
|Alternative Names||Cathepsin L|Cathepsin L; cathepsin L1; CATL; CTSL; CTSL1; CTSLEC 22.214.171.124; EC 3.4.22; FLJ31037; Major excreted protein; MEP|
|Post Tranlational Modifications||Cleavage; Phosphorylation; Glycosylation; Reduction; Methylation; Carboxylation; Oxidation; Nitration; Biotinylation; Phosphorothioation; Ubiquitination|
|Gene Location||17p14, on Chromasome 17, gene sequence:|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For Ctsl (Source: Uniprot.org, NCBI)
|Protein Function||Important for the overall degradation of proteins in lysosomes. Procathepsin L is required for maximal stimulation of steroidogenesis by TIMP1.|
|Tissue Specificity||Both mature cathepsin L1 and procathepsin L are found in the upper epidermis. The lower epidermis predominantly contains procathepsin L. In seminiferous tubules expression is greater at stages VI-VII than at stages IX-XII.|
|Related Diseases||Neoplasms; Malignant Neoplasms; Carcinoma; Cell Invasion; Neoplasm Metastasis; Malignant Paraganglionic Neoplasm; Neoplasm Invasiveness; Arthritis; Malignant Squamous Cell Neoplasm; Malignant Neoplasm Of Breast; Mammary Neoplasms; Rheumatoid Arthritis; Adenocarcinoma; Inflammation; Tumor Angiogenesis; Lung Neoplasms; Tumor Progression; Melanoma; Cell Transformation, Neoplastic; Colorectal Neoplasms|
|Related Pathways||Proteolysis; Secretion; Localization; Pathogenesis; Bone Resorption; Angiogenesis; Cell Death; Translation; Transport; Autophagy; Cell Proliferation; Glycosylation; Cell Adhesion; Tissue Remodeling; Membrane Fusion; Antigen Processing And Presentation; Reverse Transcription; Ovulation; Immune Response; Phagocytosis|
|Background||Cathepsin L is a lysosomal cysteine proteinase with a major role in intracellular protein catabolism. It also shows the most potent collagenolytic and elastinolytic activity in vitro of any of the cathepsins. Cathepsin L has been implicated in pathologic processes including myofibril necrosis in myopathies and in myocardial ischemia, and in the renal tubular response to proteinuria. Human liver cathepsin L consists of a heavy chain of about 25 kD and a light chain of about 5 kD. The gene is mapped to 9q21-q22. Cathepsin L is required for endothelial progenitor cell-induced neovascularization.|
|Scientific References||PMID: 3666143 by Ishidoh K., et al. Molecular cloning and sequencing of cDNA for rat cathepsin L.|
PMID: 2599113 by Ishidoh K., et al. Gene structure and 5'-upstream sequence of rat cathepsin L.
Our Boster Quality Guarantee for Anti-Cathepsin L Ctsl Antibody (Monoclonal, 33/2) covers its use in the following applications.Immunohistochemistry(Frozen Section), 4μg/ml, Human, mouse, rat, -
Western blot, 2μg/ml, Human, mouse, rat
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
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