radio
SKU MA1014
Size 100μg/vial
Reactivity Human, Mouse, Rat
Clonality Monoclonal
Clone Number 33/2
Host Mouse
Ig Isotype Mouse IgG1
Applications IHC, WB

Overview

Product Name Anti-Cathepsin L Antibody (Monoclonal, 33/2)
SKU/Catalog Number MA1014
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size 100μg/vial
Description Mouse IgG monoclonal antibody for Cathepsin L, cathepsin L1 (CTSL1) detection. Tested with WB, IHC-F in Human;mouse;rat. No cross reactivity with other proteins.
Cite This Product Anti-Cathepsin L Antibody (Monoclonal, 33/2) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1014)
Host Mouse
Immunogen Procathepsin L isolated from the human lung cancer cell line EPLC 32M1.
Reactivity Human, Mouse, Rat

Assay Details

Assay Dilutions Overview

Immunohistochemistry(Frozen Section), 4μg/ml, Human, mouse, rat, -
Western blot, 2μg/ml, Human, mouse, rat

Boster's Secondary Antibodies And IHC, WB Kits

The following reagents are used to generate the images below.

Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(F).

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id P07154
Gene Name Ctsl
Protein Name Cathepsin L1
Tissue Specificity Both mature cathepsin L1 and procathepsin L are found in the upper epidermis. The lower epidermis predominantly contains procathepsin L. In seminiferous tubules expression is greater at stages VI-VII than at stages IX-XII. .
Alternative Names Cathepsin L1;3.4.22.15;Cathepsin L;Cyclic protein 2;CP-2;Major excreted protein;MEP;Procathepsin L;Cathepsin L1 heavy chain;Cathepsin L1 light chain;Ctsl;Ctsl1;
Subcellular Localization Lysosome .
Molecular Weight 37660 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Important for the overall degradation of proteins in lysosomes. Procathepsin L is required for maximal stimulation of steroidogenesis by TIMP1. .
Background Cathepsin L is a lysosomal cysteine proteinase with a major role in intracellular protein catabolism. It also shows the most potent collagenolytic and elastinolytic activity in vitro of any of the cathepsins. Cathepsin L has been implicated in pathologic processes including myofibril necrosis in myopathies and in myocardial ischemia, and in the renal tubular response to proteinuria. Human liver cathepsin L consists of a heavy chain of about 25 kD and a light chain of about 5 kD. The gene is mapped to 9q21-q22. Cathepsin L is required for endothelial progenitor cell-induced neovascularization.

Order Product (MA1014)

Promotion:

Buy primary get secondary antibody for free.
Option Price
30ug sample size $99
100ug $280
100ug+Free HRP Secondary BA1050 $280
100ug+Free Biotin Secondary BA1001 $280
Ships in 5-7 business days.

Troubleshooting

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Customer Q&As

Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.