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Product Info Summary
| SKU: | A00130-2 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, WB, IHC-P-Leica |
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Product info
Product Name
Anti-CBS Antibody (Center)
SKU/Catalog Number
A00130-2
Size
80 µl
Form
Liquid
Description
Boster Bio Anti-CBS Antibody (Center) (Catalog # A00130-2). Tested in WB, IHC-P-Leica, Flow Cytometry, IF application(s). This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-CBS Antibody (Center) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00130-2)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This CBS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 301-330 amino acids from the Central region of human CBS.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00130-2 is reactive to CBS in Human, Mouse, Rat
Calculated molecular weight
60.6 kDa
Background of CBS
CBS acts as a homotetramer to catalyze the conversion of homocysteine to cystathionine, the first step in the transsulfuration pathway. This protein is allosterically activated by adenosyl-methionine and uses pyridoxal phosphate as a cofactor. Defects in this gene can cause cystathionine beta-synthase deficiency (CBSD), which can lead to homocystinuria.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00130-2 is guaranteed for Flow Cytometry, IF, WB, IHC-P-Leica Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2000
IF: 1:25
IHC-P-Leica: 1:500
FC: 1:25
Validation Images & Assay Conditions
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All lanes : Anti-CBS Antibody (Center) at 1:500 dilution
Lane 1: Hela whole cell lysate
Lane 2: MCF-7 whole cell lysate
Lane 3: LNCap whole cell lysate
Lane 4: mouse liver lysate
Lane 5: mouse pancreas lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 61 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling CBS with A00130-2 at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm and nucleus staining on HeLa cell line. The nuclear counter stain is DAPI (blue).
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All lanes : Anti-CBS Antibody (Center) at 1:2000 dilution
Lane 1: Raji whole cell lysate
Lane 2: Hela whole cell lysate
Lane 3: MCF-7 whole cell lysate
Lane 4: LNCaP whole cell lysate
Lane 5: Human liver lysate
Lane 6: Mouse liver lysate
Lane 7: Mouse pancreas lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 61 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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Immunohistochemical analysis of A00130-2 on paraffin-embedded Human liver tissue was performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:500) for 15min at room temperature. Leica Bond Polymer Refine Detection was used as the secondary antibody.
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Immunohistochemical analysis of A00130-2 on paraffin-embedded Human brain tissue was performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:500) for 15min at room temperature. Leica Bond Polymer Refine Detection was used as the secondary antibody.
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Overlay histogram showing Hela cells stained with A00130-2 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (A00130-2, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1g/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Specific Publications For Anti-CBS Antibody (Center) (A00130-2)
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