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Ccl7 antibody (A02379-1) performs well in ELISA, WB and shows reactivity with Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Western blot analysis of Ccl7 using anti-Ccl7 antibody (A02379-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: recombinant rat Ccl7 protein 10 ng,
Lane 2: recombinant rat Ccl7 protein 5 ng.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Ccl7 antigen affinity purified polyclonal antibody (Catalog # A02379-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Ccl7 at approximately 9 kDa.
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: recombinant rat Ccl7 protein 10 ng,
Lane 2: recombinant rat Ccl7 protein 5 ng.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Ccl7 antigen affinity purified polyclonal antibody (Catalog # A02379-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Ccl7 at approximately 9 kDa.
Designing a Western blot for CCL7? The guide covers the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.
Open the CCL7 Western Blot GuideBundle Save
Anti-Ccl7 Antibody Picoband®
Ccl7 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Rat | Rabbit | ELISA, WB |
Tested Applications
Ccl7 antibody was positively detected by WB in:
recombinant rat Ccl7 protein
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Rat
ELISA
0.1-0.5 μg/ml
Rat
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-Ccl7 Antibody Picoband®
View all CCL7 Antibodies
View all CCL7 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt.
After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months.
Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived rat Ccl7 recombinant protein (Position: Q24-P97).
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
9 kDa
Calculated Molecular Weight
10.9 kDa
Background of Ccl7
Chemokine (C-C motif) ligand 7 (CCL7) is a small cytokine known as a chemokine that was previously called monocyte-specific chemokine 3 (MCP3). It belongs to the C-C chemokine family. By fluorescence in situ hybridization, MCP3 gene is mapped to chromosome 17q11.2-q12. MCP3 was identified as a physiologic substrate of gelatinase A. Cleaved MCP3 binds to CC-chemokine receptors-1, -2, and -3, but no longer induces calcium fluxes or promotes chemotaxis, and instead acts as a general chemokine antagonist that dampens inflammation, it has been found that matrix metalloproteinases are both effectors and regulators of the inflammatory response.
Cite This Product
Anti-Ccl7 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02379-1)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Rat
100%
High Homology
Mouse
95%
High Homology
Dog
62%
Low homology
Monkey
62%
Low homology
Human
59%
Low homology
Cow
55%
Low homology
Sheep
54%
Low homology
Zebrafish
32%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for Ccl7 antibody
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Product Promise
We promise Anti-Ccl7 Antibody Picoband® performs excellently in relevant applications for ELISA, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A02379-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 19, 2026