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Product Info Summary
| SKU: | PB9549 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-CCN1/CYR61 Antibody Picoband®
SKU/Catalog Number
PB9549
PB0559 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CCN1/CYR61 Antibody Picoband® catalog # PB9549. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CCN1/CYR61 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9549)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human CCN1 recombinant protein (Position: K54-D381). Human CCN1 shares 91.8% and 92.1% amino acid (aa) sequence identity with mouse and rat CCN1, respectively.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9549 is reactive to CYR61 in Human, Mouse, Rat
Observed Molecular Weight
42 kDa
Calculated molecular weight
42.0 kDa
Background of CYR61
Cysteine-rich angiogenic inducer 61 (CYR61) or CCN family member 1 (CCN1), is a matricellular protein that in humans is encoded by the CYR61 gene. CYR61 is a secreted, extracellular matrix (ECM)-associated signaling protein of the CCN family (CCN intercellular signaling protein). And it is capable of regulating a broad range of cellular activities, including cell adhesion, migration, proliferation, differentiation, apoptosis, and senescence through interaction with cell surface integrin receptors and heparan sulfate proteoglycans. During embryonic development, CYR61 is critical for cardiac septal morphogenesis, blood vessel formation in placenta, and vascular integrity. In adulthood CYR61 plays important roles in inflammation and tissue repair, and is associated with diseases related to chronic inflammation, including rheumatoid arthritis, atherosclerosis, diabetes-related nephropathy and retinopathy, and many different forms of cancers.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9549 is guaranteed for IHC, WB Boster Guarantee
Recommend Dilution
| Application | Dilution | Species |
|---|---|---|
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat |
| Immunohistochemistry (Paraffin-embedded Section) | 2-5μg/ml | Human |
Tested application
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 µg per lane
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Validation Images & Assay Conditions
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Western blot analysis of CCN1/CYR61 using anti-CCN1/CYR61 antibody (PB9549).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SiHa whole cell lysates,
Lane 2: human U251 whole cell lysates,
Lane 3: human U2OS whole cell lysates,
Lane 4: human A549 whole cell lysates,
Lane 5: rat lung tissue lysates,
Lane 6: rat kidney tissue lysates,
Lane 7: mouse kidney tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCN1/CYR61 antigen affinity purified polyclonal antibody (PB9549) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for CCN1/CYR61 at approximately 42 kDa. The expected band size for CCN1/CYR61 is at 42 kDa.
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IHC analysis of CCN1/CYR61 using anti-CCN1/CYR61 antibody (PB9549).
CCN1/CYR61 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CCN1/CYR61 Antibody (PB9549) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Specific Publications For Anti-CCN1/CYR61 Antibody Picoband® (PB9549)
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Customer Q&As
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2 Customer Q&As for Anti-CCN1/CYR61 Antibody Picoband®
Question
Which amino acids or protein structure PB9549 binds to?
Verified customer
Asked: 2021-02-12
Answer
The immunogen sequence for the Anti-CCN1/CYR61 Antibody Picoband™ (PB9549) is K54-D381.
Boster Scientific Support
Answered: 2021-02-16
Question
We are currently using anti-CCN1/CYR61 antibody PB9549 for rat tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, rat. Is it possible that the antibody can work on goat tissues as well?
M. Rodriguez
Verified customer
Asked: 2013-08-02
Answer
The anti-CCN1/CYR61 antibody (PB9549) has not been tested for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-08-02
