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Product Info Summary
| SKU: | M01748 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-CCR3 Rabbit Monoclonal Antibody
SKU/Catalog Number
M01748
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-CCR3 Rabbit Monoclonal Antibody catalog # M01748. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CCR3 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01748)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ECE-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human CCR3
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01748 is reactive to CCR3 in Human, Mouse, Rat
Applications
M01748 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
43 kDa, 32 kDa, 29 kDa
Calculated molecular weight
41.044kDa
Background of CCR3
Furin is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX (K/R) R consensus motif.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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Western blot analysis of CCR3 expression in K562 cell lysate.
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Immunohistochemical analysis of paraffin-embedded human epidemal cells of skin, using CCR3 Antibody .
Protein Target Info & Infographic
Gene/Protein Information For CCR3 (Source: Uniprot.org, NCBI)
Gene Name
CCR3
Full Name
C-C chemokine receptor type 3
Weight
41.044kDa
Superfamily
G-protein coupled receptor 1 family
Alternative Names
b-chemokine receptor; CC chemokine receptor 3; C-C chemokine receptor type 3; C-C CKR-3; CCR3; CCR-3; CD193 antigen; CD193; chemokine (C-C motif) receptor 3; CKR3CC-CKR-3CMKBR3CD193; eosinophil CC chemokine receptor 3; Eosinophil eotaxin receptor; MGC102841 CCR3 C C CKR3, CC-CKR-3, CD193, CKR 3, CKR3, CMKBR3 C-C motif chemokine receptor 3 C-C chemokine receptor type 3|C-C CKR-3|CC chemokine receptor 3|CCR-3|b-chemokine receptor|chemokine (C-C motif) receptor 3|eosinophil CC chemokine receptor 3|eosinophil eotaxin receptor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CCR3, check out the CCR3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CCR3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CCR3 Rabbit Monoclonal Antibody (M01748)
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No publications found for M01748
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Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
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- Bioscience Research Protocol Resources
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Customer Reviews
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*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
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Find answers in Q&As, reviews.
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16 Customer Q&As for Anti-CCR3 Rabbit Monoclonal Antibody
Question
We have observed staining in mouse synovium. Are there any suggestions? Is anti-CCR3 Rabbit Monoclonal antibody supposed to stain synovium positively?
Verified Customer
Verified customer
Asked: 2020-04-24
Answer
According to literature synovium does express CCR3. According to Uniprot.org, CCR3 is expressed in blood, monocyte, synovium, among other tissues. Regarding which tissues have CCR3 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15489334
Monocyte, Pubmed ID: 7622448
Synovium, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2020-04-24
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for blood using anti-CCR3 Rabbit Monoclonal antibody M01748. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-03-26
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-26
Question
I would like using your anti-CCR3 Rabbit Monoclonal antibody for cell chemotaxis studies. Has this antibody been tested with western blotting on k562 cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-02-12
Answer
We appreciate your inquiry. This M01748 anti-CCR3 Rabbit Monoclonal antibody is validated on k562 cell lysate, human epidemal cells. It is guaranteed to work for IF, IHC, ICC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-02-12
Question
Is a blocking peptide available for product anti-CCR3 Rabbit Monoclonal antibody (M01748)?
Verified Customer
Verified customer
Asked: 2019-12-05
Answer
We do provide the blocking peptide for product anti-CCR3 Rabbit Monoclonal antibody (M01748). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-12-05
Question
I was wanting to use your anti-CCR3 Rabbit Monoclonal antibody for WB for rat blood on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat blood identification?
Verified Customer
Verified customer
Asked: 2019-12-03
Answer
As indicated on the product datasheet, M01748 anti-CCR3 Rabbit Monoclonal antibody has been validated for IF, IHC, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat blood in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-03
Question
We have tried in the past anti-CCR3 Rabbit Monoclonal antibody for WB on synovium a few months ago. I am using rat, and We are going to use the antibody for ICC next. you antibody examining synovium as well as monocyte in our next experiment. Could you please give me some suggestion on which antibody would work the best for ICC?
Verified Customer
Verified customer
Asked: 2019-12-03
Answer
I have checked the website and datasheets of our anti-CCR3 Rabbit Monoclonal antibody and I see that M01748 has been tested on rat in both WB and ICC. Thus M01748 should work for your application. Our Boster satisfaction guarantee will cover this product for ICC in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for ICC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-12-03
Question
I see that the anti-CCR3 Rabbit Monoclonal antibody M01748 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-11-25
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-11-25
Question
Is this M01748 anti-CCR3 Rabbit Monoclonal antibody reactive to the isotypes of CCR3?
Verified Customer
Verified customer
Asked: 2019-08-23
Answer
The immunogen of M01748 anti-CCR3 Rabbit Monoclonal antibody is A synthesized peptide derived from human CCR3. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-08-23
Question
Would M01748 anti-CCR3 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-08-23
Answer
It shows on the product datasheet, M01748 anti-CCR3 Rabbit Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-08-23
Question
Please see the WB image, lot number and protocol we used for blood using anti-CCR3 Rabbit Monoclonal antibody M01748. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-07-03
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-03
Question
We were happy with the WB result of your anti-CCR3 Rabbit Monoclonal antibody. However we have observed positive staining in monocyte cell membrane using this antibody. Is that expected? Could you tell me where is CCR3 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-06-11
Answer
Based on literature, monocyte does express CCR3. Generally CCR3 expresses in cell membrane. Regarding which tissues have CCR3 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15489334
Monocyte, Pubmed ID: 7622448
Synovium, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2019-06-11
Question
We are currently using anti-CCR3 Rabbit Monoclonal antibody M01748 for human tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2018-04-13
Answer
The anti-CCR3 Rabbit Monoclonal antibody (M01748) has not been validated for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-04-13
Question
My question regards to test anti-CCR3 Rabbit Monoclonal antibody M01748 on rat blood for research purposes, then I may be interested in using anti-CCR3 Rabbit Monoclonal antibody M01748 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-01-24
Answer
The products we sell, including anti-CCR3 Rabbit Monoclonal antibody M01748, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-01-24
Question
Will anti-CCR3 Rabbit Monoclonal antibody M01748 work for WB with blood?
H. Huang
Verified customer
Asked: 2016-04-25
Answer
According to the expression profile of blood, CCR3 is highly expressed in blood. So, it is likely that anti-CCR3 Rabbit Monoclonal antibody M01748 will work for WB with blood.
Boster Scientific Support
Answered: 2016-04-25
Question
Can you help my question with product M01748, anti-CCR3 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
A. Williams
Verified customer
Asked: 2015-02-24
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01748 anti-CCR3 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2015-02-24
Question
Do you have a BSA free version of anti-CCR3 Rabbit Monoclonal antibody M01748 available?
G. Parker
Verified customer
Asked: 2013-07-02
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-CCR3 Rabbit Monoclonal antibody M01748 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2013-07-02
