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Product Info Summary
| SKU: | A30522 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, ICC, WB |
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Product info
Product Name
Anti-CCRK CDK20 Antibody
SKU/Catalog Number
A30522
Size
100ul
Form
Liquid
Description
Boster Bio Anti-CCRK CDK20 Antibody (Catalog# A30522). Tested in WB, IHC-P, IF, ICC, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CCRK CDK20 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30522)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human CCRK. Immunogen sequence location: 31-80
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A30522 is reactive to CDK20 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
72 kDa
Calculated molecular weight
38695 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A30522 is guaranteed for ELISA, IF, IHC-P, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western Blot, 1:500 - 1:2000
Immunohistochemistry, 1:100 - 1:300
Immunofluorescence, 1:200 - 1:1000
ELISA, 1:20000
Not yet tested in other applications.
Validation Images & Assay Conditions
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Western blot analysis of lysates from RAW264.7 cells, using CCRK Antibody. The lane on the right is blocked with the synthesized peptide.
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Immunofluorescence analysis of HUVEC cells, using CCRK Antibody. The picture on the right is blocked with the synthesized peptide.
Protein Target Info & Infographic
Gene/Protein Information For CDK20 (Source: Uniprot.org, NCBI)
Gene Name
CDK20
Full Name
Cyclin-dependent kinase 20
Weight
38695 MW
Superfamily
protein kinase superfamily
Alternative Names
CDK20;CCRK;CDCH;Cyclin-dependent kinase 20;CDK-activating kinase p42;CAK-kinase p42;Cell cycle-related kinase;Cell division protein kinase 20;Cyclin-dependent protein kinase H;Cyclin-kinase-activating kinase p42 CDK20 CCRK, CDCH, P42, PNQALRE cyclin dependent kinase 20 cyclin-dependent kinase 20|CAK-kinase p42|CDK-activating kinase p42|cell cycle-related kinase|cell division protein kinase 20|cyclin-dependent protein kinase H|cyclin-kinase-activating kinase p42
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CDK20, check out the CDK20 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CDK20: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CCRK CDK20 Antibody (A30522)
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No publications found for A30522
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Customer Q&As
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5 Customer Q&As for Anti-CCRK CDK20 Antibody
Question
I see that the anti-CCRK antibody A30522 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-10-17
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-10-17
Question
Will anti-CCRK antibody A30522 work on dog WB with uterus?
Verified Customer
Verified customer
Asked: 2019-08-06
Answer
Our lab technicians have not tested anti-CCRK antibody A30522 on dog. You can run a BLAST between dog and the immunogen sequence of anti-CCRK antibody A30522 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated dog samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in dog uterus in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-06
Question
We are currently using anti-CCRK antibody A30522 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on pig tissues as well?
R. Krishna
Verified customer
Asked: 2015-12-30
Answer
The anti-CCRK antibody (A30522) has not been validated for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-12-30
Question
I am interested in to test anti-CCRK antibody A30522 on mouse uterus for research purposes, then I may be interested in using anti-CCRK antibody A30522 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
K. Brown
Verified customer
Asked: 2015-06-04
Answer
The products we sell, including anti-CCRK antibody A30522, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2015-06-04
Question
Is a blocking peptide available for product anti-CCRK antibody (A30522)?
A. Roberts
Verified customer
Asked: 2013-04-29
Answer
We do provide the blocking peptide for product anti-CCRK antibody (A30522). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2013-04-29
