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Product Info Summary
| SKU: | M16124 |
|---|---|
| Size: | 50 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-CCT8L2 Antibody (N-Term)
SKU/Catalog Number
M16124
Size
50 µl
Description
Boster Bio Anti-CCT8L2 Antibody (N-Term) (Catalog # M16124). Tested in WB, Flow Cytometry application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-CCT8L2 Antibody (N-Term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M16124)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This CCT8L2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 92-124 amino acids from human CCT8L2.
Reactive Species
M16124 is reactive to CCT8L2 in Human
Calculated molecular weight
59.4 kDa
Background of CCT8L2
Possible molecular chaperone; assists the folding of proteins upon ATP hydrolysis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M16124 is guaranteed for Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2000
FC: 1:25
Validation Images & Assay Conditions
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All lanes : Anti-CCT8L2 Antibody (N-Term) at 1:2000 dilution
Lane 1: Jurkat whole cell lysate
Lane 2: CCRF-CEM whole cell lysate
Lane 3: A673 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 59 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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Overlay histogram showing Jurkat cells stained with M16124 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (M16124, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Specific Publications For Anti-CCT8L2 Antibody (N-Term) (M16124)
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