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Product Info Summary
| SKU: | A08216 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-CD153 TNFSF8 Antibody
SKU/Catalog Number
A08216
Size
100ul
Form
Liquid
Description
Boster Bio Anti-CD153 TNFSF8 Antibody (Catalog# A08216). Tested in WB, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD153 TNFSF8 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08216)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human CD153. Immunogen sequence location: 71-120
Reactive Species
A08216 is reactive to TNFSF8 in Human, Mouse, Rat
Observed Molecular Weight
72 kDa
Calculated molecular weight
26.0 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A08216 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500 - 1:2000.
ICC/IF 1:200 - 1:1000.
ELISA: 1:20000.
Not yet tested in other applications.
Validation Images & Assay Conditions
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Western blot analysis of lysates from RAW264.7 cells, using CD153 Antibody. The lane on the right is blocked with the synthesized peptide.
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Western blot analysis of the lysates from K562 cells using CD153 antibody.
Specific Publications For Anti-CD153 TNFSF8 Antibody (A08216)
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Customer Reviews
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7 Customer Q&As for Anti-CD153 TNFSF8 Antibody
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for leukocyte using anti-CD153 antibody A08216. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-03-11
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-11
Question
Do you have a BSA free version of anti-CD153 antibody A08216 available?
Verified Customer
Verified customer
Asked: 2020-02-05
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-CD153 antibody A08216 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-02-05
Question
I see that the anti-CD153 antibody A08216 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-02-04
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2020-02-04
Question
Is this A08216 anti-CD153 antibody reactive to the isotypes of TNFSF8?
Verified Customer
Verified customer
Asked: 2019-12-06
Answer
The immunogen of A08216 anti-CD153 antibody is A peptide derived from human CD153. Immunogen sequence location: 71-120. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-12-06
Question
I have a question about product A08216, anti-CD153 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-11-12
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A08216 anti-CD153 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-11-12
Question
Does A08216 anti-CD153 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
G. Baker
Verified customer
Asked: 2016-08-22
Answer
As indicated on the product datasheet, A08216 anti-CD153 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2016-08-22
Question
I was wanting to use your anti-CD153 antibody for WB for mouse leukocyte on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse leukocyte identification?
P. Gonzalez
Verified customer
Asked: 2016-01-20
Answer
It shows on the product datasheet, A08216 anti-CD153 antibody has been tested for WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse leukocyte in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-01-20
