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Product Info Summary
| SKU: | A07017 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-CD158z KIR3DL3 Antibody
SKU/Catalog Number
A07017
Size
100μl
Form
Liquid
Description
Boster Bio Anti-CD158z KIR3DL3 Antibody catalog # A07017. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD158z KIR3DL3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07017)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from the Internal region of human KIR3DL3. AA range:231-280
Reactive Species
A07017 is reactive to KIR3DL3 in Human
Observed Molecular Weight
39 kDa
Calculated molecular weight
44.9 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07017 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC: 1:100-300
ELISA 1:20000
IF 1:50-200
Validation Images & Assay Conditions
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Western Blot (WB) analysis of K562, L929, A549 cells using CD158z Polyclonal antibody.
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Immunohistochemistry (IHC) analysis of paraffin-embedded Human Brain, antibody was diluted at 1:100.
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Immunohistochemistry (IHC) analysis of paraffin-embedded Human Brain, antibody was diluted at 1:100.
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Immunohistochemistry (IHC) analysis of paraffin-embedded Human Lung Cancer, antibody was diluted at 1:100.
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Immunohistochemistry (IHC) analysis of paraffin-embedded Human Lung Cancer, antibody was diluted at 1:100.
Specific Publications For Anti-CD158z KIR3DL3 Antibody (A07017)
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-CD158z KIR3DL3 Antibody
Question
We need to test anti-CD158z antibody A07017 on human peripheral blood {eco:0000312 for research purposes, then I may be interested in using anti-CD158z antibody A07017 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Z. Parker
Verified customer
Asked: 2020-04-28
Answer
The products we sell, including anti-CD158z antibody A07017, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-04-28
Question
We are currently using anti-CD158z antibody A07017 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-07
Answer
The anti-CD158z antibody (A07017) has not been validated for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-07
Question
I was wanting to use your anti-CD158z antibody for WB for human peripheral blood eco:0000312 on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been validated and is this antibody a good choice for human peripheral blood {eco:0000312 identification?
Verified Customer
Verified customer
Asked: 2020-01-17
Answer
As indicated on the product datasheet, A07017 anti-CD158z antibody has been validated for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human peripheral blood {eco:0000312 in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-17
Question
My question regarding product A07017, anti-CD158z antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-10-09
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A07017 anti-CD158z antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-10-09
Question
Is a blocking peptide available for product anti-CD158z antibody (A07017)?
Verified Customer
Verified customer
Asked: 2018-04-06
Answer
We do provide the blocking peptide for product anti-CD158z antibody (A07017). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2018-04-06
Question
I have attached the WB image, lot number and protocol we used for peripheral blood {eco:0000312 using anti-CD158z antibody A07017. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-02-05
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-02-05
Question
I see that the anti-CD158z antibody A07017 works with WB, what is the protocol used to produce the result images on the product page?
R. Miller
Verified customer
Asked: 2018-01-22
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2018-01-22
