Click image to see more details
Product Info Summary
| SKU: | M00812 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IP, IHC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-CD163 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00812
BM5113 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-CD163 Rabbit Monoclonal Antibody catalog # M00812. Tested in WB, IHC, IP applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD163 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00812)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ABCC-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human CD163
Reactive Species
M00812 is reactive to CD163 in Human
Observed Molecular Weight
150 kDa
Calculated molecular weight
125.5 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00812 is guaranteed for IP, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
IP 1:30
Positive Control
WB: Human fetal kidney
IHC: mouse liver tissue
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of CD163 expression in Human fetal kidney lysate.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded mouse liver, using CD163 Antibody.
Specific Publications For Anti-CD163 Rabbit Monoclonal Antibody (M00812)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-CD163 Rabbit Monoclonal Antibody?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-CD163 Rabbit Monoclonal Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
16 Customer Q&As for Anti-CD163 Rabbit Monoclonal Antibody
Question
What is the concentration of M00812 in each vial?
Verified customer
Asked: 2021-02-25
Answer
The concentration of the Anti-CD163 Rabbit Monoclonal Antibody (M00812) in each vial is 1mg/ml.
Boster Scientific Support
Answered: 2021-02-25
Question
Do you have a BSA free version of anti-CD163 Rabbit Monoclonal antibody M00812 available?
Verified Customer
Verified customer
Asked: 2020-05-04
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CD163 Rabbit Monoclonal antibody M00812 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-05-04
Question
I would like to test anti-CD163 Rabbit Monoclonal antibody M00812 on human esophagus for research purposes, then I may be interested in using anti-CD163 Rabbit Monoclonal antibody M00812 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-12-20
Answer
The products we sell, including anti-CD163 Rabbit Monoclonal antibody M00812, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-12-20
Question
We purchased anti-CD163 Rabbit Monoclonal antibody for WB on plasma a few months ago. I am using human, and We want to use the antibody for IP next. My lab would like examining plasma as well as liver in our next experiment. Could give a recommendation on which antibody would work the best for IP?
Verified Customer
Verified customer
Asked: 2019-10-31
Answer
I looked at the website and datasheets of our anti-CD163 Rabbit Monoclonal antibody and it appears that M00812 has been tested on human in both WB and IP. Thus M00812 should work for your application. Our Boster satisfaction guarantee will cover this product for IP in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IP detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-10-31
Question
We have been able to see staining in human spleen. Are there any suggestions? Is anti-CD163 Rabbit Monoclonal antibody supposed to stain spleen positively?
Verified Customer
Verified customer
Asked: 2019-09-30
Answer
According to literature spleen does express CD163. According to Uniprot.org, CD163 is expressed in esophagus, spleen, plasma, liver, among other tissues. Regarding which tissues have CD163 expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 19159218, 24275569
Plasma, Pubmed ID: 16335952
Spleen, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-09-30
Question
Would anti-CD163 Rabbit Monoclonal antibody M00812 work for IP with esophagus?
Verified Customer
Verified customer
Asked: 2019-08-01
Answer
According to the expression profile of esophagus, CD163 is highly expressed in esophagus. So, it is likely that anti-CD163 Rabbit Monoclonal antibody M00812 will work for IP with esophagus.
Boster Scientific Support
Answered: 2019-08-01
Question
We are currently using anti-CD163 Rabbit Monoclonal antibody M00812 for human tissue, and we are content with the IP results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-01
Answer
The anti-CD163 Rabbit Monoclonal antibody (M00812) has not been tested for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-01
Question
I have attached the WB image, lot number and protocol we used for esophagus using anti-CD163 Rabbit Monoclonal antibody M00812. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-05-03
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-03
Question
Is a blocking peptide available for product anti-CD163 Rabbit Monoclonal antibody (M00812)?
Verified Customer
Verified customer
Asked: 2019-02-27
Answer
We do provide the blocking peptide for product anti-CD163 Rabbit Monoclonal antibody (M00812). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-02-27
Question
Our team were satisfied with the WB result of your anti-CD163 Rabbit Monoclonal antibody. However we have observed positive staining in liver soluble cd163: secreted using this antibody. Is that expected? Could you tell me where is CD163 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2018-11-26
Answer
From literature, liver does express CD163. Generally CD163 expresses in soluble cd163: secreted, cell membrane. Regarding which tissues have CD163 expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 19159218, 24275569
Plasma, Pubmed ID: 16335952
Spleen, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2018-11-26
Question
Is this M00812 anti-CD163 Rabbit Monoclonal antibody reactive to the isotypes of CD163?
J. Miller
Verified customer
Asked: 2018-11-09
Answer
The immunogen of M00812 anti-CD163 Rabbit Monoclonal antibody is A synthesized peptide derived from human CD163. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-11-09
Question
I see that the anti-CD163 Rabbit Monoclonal antibody M00812 works with IP, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-07-03
Answer
You can find protocols for IP on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2018-07-03
Question
Can you help my question with product M00812, anti-CD163 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-09-25
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00812 anti-CD163 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-09-25
Question
I was wanting to use your anti-CD163 Rabbit Monoclonal antibody for IP for human esophagus on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human esophagus identification?
P. Jones
Verified customer
Asked: 2016-06-27
Answer
As indicated on the product datasheet, M00812 anti-CD163 Rabbit Monoclonal antibody has been validated for IP, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human esophagus in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-06-27
Question
Would M00812 anti-CD163 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
S. Singh
Verified customer
Asked: 2014-11-24
Answer
You can see on the product datasheet, M00812 anti-CD163 Rabbit Monoclonal antibody as been validated on IP. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2014-11-24
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for esophagus using anti-CD163 Rabbit Monoclonal antibody M00812. Let me know if you need anything else.
K. Carter
Verified customer
Asked: 2013-11-01
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-11-01
