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Product Info Summary
| SKU: | A00154 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC-P, WB |
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Product info
Product Name
Anti-CD19 Antibody (N-term)
SKU/Catalog Number
A00154
Size
80 µl
Form
Liquid
Description
Boster Bio Anti-CD19 Antibody (N-term) (Catalog # A00154). Tested in WB, IHC-P, Flow Cytometry, IF application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-CD19 Antibody (N-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00154)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This CD19 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 143-172 amino acids from the N-terminal region of human CD19.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00154 is reactive to CD19 in Human
Calculated molecular weight
61.1 kDa
Background of CD19
Lymphocytes proliferate and differentiate in response to various concentrations of different antigens. The ability of the B cell to respond in a specific, yet sensitive manner to the various antigens is achieved with the use of low-affinity antigen receptors. CD19 is a cell surface molecule which assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00154 is guaranteed for Flow Cytometry, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IF: 1:10-1:50
WB: 1:500
IHC-P: 1:10-1:50
FC: 1:10-1:50
Validation Images & Assay Conditions
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Immunofluorescence analysis of CD19 Antibody (N-term) with paraffin-embedded human lymph tissue . 0. 05 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence. Red counterstaining is PI.
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CD19 Antibody (N-term) (Cat. #A00154) western blot analysis in Raji cell line lysates (35ug/lane). This demonstrates the CD19 antibody detected the CD19 protein (arrow).
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Western blot analysis of CD19 (arrow) using rabbit polyclonal CD19 Antibody (N-term) (Cat. #A00154). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CD19 gene (Lane 2) (Origene Technologies).
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Western blot analysis of lysate from Ramos cell line, using CD19Antibody (N-term) (Cat. #A00154). A00154 was diluted at 1:500. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.
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Formalin-fixed and paraffin-embedded human lymph reacted with CD19 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Flow cytometric analysis of CEM cells using CD19 Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Specific Publications For Anti-CD19 Antibody (N-term) (A00154)
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