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- Table of Contents
MS4A1 antibody (M03780-4) performs well in IF, WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 1 Publication
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IF analysis of CD20/MS4A1 using anti-CD20/MS4A1 antibody (M03780-4).
CD20/MS4A1 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-CD20/MS4A1 Antibody (M03780-4) overnight at 4°C. Biotin conjugated goat anti-mouse IgG (BA1001) was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using DyLight®550 Conjugated Avidin (BA1134). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
CD20/MS4A1 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-CD20/MS4A1 Antibody (M03780-4) overnight at 4°C. Biotin conjugated goat anti-mouse IgG (BA1001) was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using DyLight®550 Conjugated Avidin (BA1134). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Anti-CD20/MS4A1 Antibody Picoband® (monoclonal, 4I11)
MS4A1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Mouse | IF, WB |
Tested Applications
MS4A1 antibody was positively detected by WB in:
human Raji whole cell, human Jurkat whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
MS4A1 antibody was positively detected by IF in:
human tonsil tissue
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Human
Immunofluorescence
5 μg/ml
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
Human
Tested
Recommend
Tested
Monkey
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-CD20/MS4A1 Antibody Picoband® (monoclonal, 4I11)
View all MS4A1 Antibodies
View all MS4A1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Host
Mouse
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Monoclonal
Clone Number
4I11
Isotype
Mouse IgG2a
Immunogen
E.coli-derived human CD20/MS4A1 recombinant protein (Position: M1-P297).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
37 kDa
Calculated Molecular Weight
33.1 kDa
Background of MS4A1
CD20, also known as MS4A1, is an activated-glycosylated phosphoprotein expressed on the surface of all B-cells beginning at the pro-B phase (CD45R+, CD117+) and progressively increasing in concentration until maturity. It is mapped to 11q12.2. This gene encodes a member of the membrane-spanning 4A gene family. The function of CD20 is to enable optimal B-cell immune response, specifically against T-independent antigens. It is suspected that CD20 acts as a calcium channel in the cell membrane. What’s more, this protein may be involved in the regulation of B-cell activation and proliferation.
Cite This Product
Anti-CD20/MS4A1 Antibody Picoband® (monoclonal, 4I11) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03780-4)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
97%
High Homology
Rabbit
80%
Moderate Homology
Rat
77%
Moderate Homology
Dog
74%
Moderate Homology
Mouse
73%
Moderate Homology
Cow
71%
Moderate Homology
Sheep
71%
Moderate Homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for MS4A1 antibody
Immunofluorescence (IF) protocol for MS4A1 antibody
Publications
Application:
Species:
Establishing prognostic models for intrahepatic cholangiocarcinoma based on immune cells
Published: 2024-10-15
Journal: World Journal of Gastrointestinal Oncology
Application: IF
Impact Factor: 2.50
Species: Human
PMID: 39473952
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Product Promise
We promise Anti-CD20/MS4A1 Antibody Picoband® (monoclonal, 4I11) performs excellently in relevant applications for IF, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
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M03780-4
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 7, 2026