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Product Info Summary
| SKU: | M01572-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-CD22/Siglec 2 Rabbit Monoclonal Antibody
SKU/Catalog Number
M01572-1
BM4178 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-CD22/Siglec 2 Rabbit Monoclonal Antibody catalog # M01572-1. Tested in WB, IHC application. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD22/Siglec 2 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01572-1)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
CBO-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human CD22
Reactive Species
M01572-1 is reactive to CD22 in Human, Mouse, Rat
Observed Molecular Weight
140 kDa
Calculated molecular weight
95.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01572-1 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
Positive Control
WB: Raji cell
Validation Images & Assay Conditions
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Western blot analysis of CD22 using anti-CD22 antibody (M01572-1).
Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Raji whole cell lysates,
Lane 2: human Ramos whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD22 antigen affinity purified monoclonal antibody (M01572-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for CD22 at approximately 140 kDa. The expected band size for CD22 is at 95 kDa.
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IHC analysis of CD22 using anti-CD22 antibody (M01572-1).
CD22 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-CD22 Antibody (M01572-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Specific Publications For Anti-CD22/Siglec 2 Rabbit Monoclonal Antibody (M01572-1)
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4 Customer Q&As for Anti-CD22/Siglec 2 Rabbit Monoclonal Antibody
Question
My colleagues were happy with the WB result of your anti-CD22/Siglec 2 Rabbit Monoclonal antibody. However we have seen positive staining in corpus callosum cell membrane using this antibody. Is that expected? Could you tell me where is CD22 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-01-02
Answer
From literature, corpus callosum does express CD22. Generally CD22 expresses in cell membrane. Regarding which tissues have CD22 expression, here are a few articles citing expression in various tissues:
Spleen, Pubmed ID: 15057824
Tonsil, Pubmed ID: 1985119
Boster Scientific Support
Answered: 2020-01-02
Question
We want using your anti-CD22/Siglec 2 Rabbit Monoclonal antibody for immunoregulatory interactions between a lymphoid and a non-lymphoid cell studies. Has this antibody been tested with western blotting on raji cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-10-10
Answer
We appreciate your inquiry. This M01572-1 anti-CD22/Siglec 2 Rabbit Monoclonal antibody is validated on raji cell lysate. It is guaranteed to work for WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-10-10
Question
We are currently using anti-CD22/Siglec 2 Rabbit Monoclonal antibody M01572-1 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2019-09-04
Answer
The anti-CD22/Siglec 2 Rabbit Monoclonal antibody (M01572-1) has not been validated for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-04
Question
We have been able to see staining in human spleen. Do you have any suggestions? Is anti-CD22/Siglec 2 Rabbit Monoclonal antibody supposed to stain spleen positively?
Verified Customer
Verified customer
Asked: 2018-10-16
Answer
Based on literature spleen does express CD22. Based on Uniprot.org, CD22 is expressed in corpus callosum, tonsil, spleen, among other tissues. Regarding which tissues have CD22 expression, here are a few articles citing expression in various tissues:
Spleen, Pubmed ID: 15057824
Tonsil, Pubmed ID: 1985119
Boster Scientific Support
Answered: 2018-10-16
