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Product Info Summary
| SKU: | M04237 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-CD23 FCER2 Monoclonal Antibody
SKU/Catalog Number
M04237
BM5465 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-CD23 FCER2 Monoclonal Antibody catalog # M04237. Tested in WB, IHC, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD23 FCER2 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04237)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ADIC-6
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human CD23 This receptor has essential roles in the regulation of IgE production and in the differentiation of B-cells (it is a B-cell-specific antigen) .
Reactive Species
M04237 is reactive to FCER2 in Human
Observed Molecular Weight
36 kDa
Calculated molecular weight
36.5 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M04237 is guaranteed for Flow Cytometry, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
FC 1:30
Positive Control
WB: Raji cell
Validation Images & Assay Conditions
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Western blot analysis of CD23 expression in Raji cell lysate.
Specific Publications For Anti-CD23 FCER2 Monoclonal Antibody (M04237)
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4 Customer Q&As for Anti-CD23 FCER2 Monoclonal Antibody
Question
See attached the WB image, lot number and protocol we used for b-cell using anti-CD23 Monoclonal antibody M04237. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-10-24
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-24
Question
I have a question about product M04237, anti-CD23 Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-05-28
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M04237 anti-CD23 Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-05-28
Question
Is a blocking peptide available for product anti-CD23 Monoclonal antibody (M04237)?
M. Moore
Verified customer
Asked: 2018-05-24
Answer
We do provide the blocking peptide for product anti-CD23 Monoclonal antibody (M04237). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2018-05-24
Question
I am looking for to test anti-CD23 Monoclonal antibody M04237 on human b-cell for research purposes, then I may be interested in using anti-CD23 Monoclonal antibody M04237 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-03-22
Answer
The products we sell, including anti-CD23 Monoclonal antibody M04237, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-03-22
