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Product Info Summary
| SKU: | A30786 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC |
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Product info
Product Name
Anti-CD234 ACKR1 Antibody
SKU/Catalog Number
A30786
Size
100ul
Form
Liquid
Description
Boster Bio Anti-CD234 ACKR1 Antibody (Catalog# A30786). Tested in ICC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD234 ACKR1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30786)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human DARC. Immunogen sequence location: 18264
Reactive Species
A30786 is reactive to ACKR1 in Human, Mouse, Rat
Observed Molecular Weight
72 kDa
Calculated molecular weight
35.6 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A30786 is guaranteed for ELISA, IF, ICC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ICC/IF 1:200 - 1:1000.
ELISA: 1:40000.
Not yet tested in other applications.
Validation Images & Assay Conditions
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Immunofluorescence analysis of A549 cells, using CD234 Antibody. The picture on the right is blocked with the synthesized peptide.
Specific Publications For Anti-CD234 ACKR1 Antibody (A30786)
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Customer Q&As
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7 Customer Q&As for Anti-CD234 ACKR1 Antibody
Question
Is a blocking peptide available for product anti-CD234 antibody (A30786)?
Verified Customer
Verified customer
Asked: 2020-03-12
Answer
We do provide the blocking peptide for product anti-CD234 antibody (A30786). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2020-03-12
Question
We need to test anti-CD234 antibody A30786 on human bone marrow for research purposes, then I may be interested in using anti-CD234 antibody A30786 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
A. Wu
Verified customer
Asked: 2019-10-22
Answer
The products we sell, including anti-CD234 antibody A30786, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-10-22
Question
Would anti-CD234 antibody A30786 work for IF with bone marrow?
Verified Customer
Verified customer
Asked: 2019-07-01
Answer
According to the expression profile of bone marrow, ACKR1 is highly expressed in bone marrow. So, it is likely that anti-CD234 antibody A30786 will work for IF with bone marrow.
Boster Scientific Support
Answered: 2019-07-01
Question
We are currently using anti-CD234 antibody A30786 for human tissue, and we are well pleased with the IF results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2019-03-28
Answer
The anti-CD234 antibody (A30786) has not been validated for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-03-28
Question
Would A30786 anti-CD234 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
T. Collins
Verified customer
Asked: 2018-08-23
Answer
It shows on the product datasheet, A30786 anti-CD234 antibody as been validated on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-08-23
Question
I was wanting to use your anti-CD234 antibody for IF for human bone marrow on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human bone marrow identification?
Verified Customer
Verified customer
Asked: 2017-11-14
Answer
It shows on the product datasheet, A30786 anti-CD234 antibody has been tested for IF on human tissues. We have an innovator award program that if you test this antibody and show it works in human bone marrow in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-11-14
Question
Would anti-CD234 antibody A30786 work on horse IF with lung?
M. Lewis
Verified customer
Asked: 2016-06-24
Answer
Our lab technicians have not validated anti-CD234 antibody A30786 on horse. You can run a BLAST between horse and the immunogen sequence of anti-CD234 antibody A30786 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse lung in IF, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-06-24
