Anti-CD28 Antibody

CD28 antibody

Boster Bio Anti-CD28 Antibody (Catalog # A00065). Tested in ELISA, WB, IHC, IF applications. This antibody reacts with Human.

Product Info Summary

SKU: A00065
Size: 0.1 mg
Reactive Species: Human
Host: Rabbit
Application: ELISA, IF, IHC, WB

Product Name

Anti-CD28 Antibody

View all CD28 Antibodies

SKU/Catalog Number

A00065

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-CD28 Antibody (Catalog # A00065). Tested in ELISA, WB, IHC, IF applications. This antibody reacts with Human.

Storage & Handling

CD28 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-CD28 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00065)

Host

Rabbit

Contents

CD28 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Clone Number

SK7

Isotype

IgG

Immunogen

CD28 antibody was raised against a peptide corresponding to 18 amino acids near the carboxy terminus of human CD28. The immunogen is located within the last 50 amino acids of CD28.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

ESR1 antibody is human, mouse and rat reactive.

Reactive Species

A00065 is reactive to CD28 in Human

Applications

A00065 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

25.066kDa

Background of CD28

Estrogen receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of ligand-activated transcription factors (1). Estrogen receptors, including ESR1, also known as ER-alpha and ESR2 (ER-beta), contain DNA binding and ligand binding domains and are critically involved in regulating the normal function of reproductive tissues. ESR1 is a widely expressed nuclear protein and serves as a strong activator of estrogen responsive genes (1,2). Phosphorylation of serines 104 and 106, located in the N-terminal transcription activation function-1 domain (AF-1), plays a large role in regulating ER alpha activity (3).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

CD28 antibody can be used for Western blot at 1μg/mL. Antibody can also be used for Immunohistochemistry at 2 μg/mL. For Immunoflorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

Gene/Protein Information For CD28 (Source: Uniprot.org, NCBI)

Gene Name

CD28

Full Name

T-cell-specific surface glycoprotein CD28

Weight

25.066kDa

Alternative Names

CD28 antigen (Tp44); CD28 antigen; CD28 molecule; CD28; MGC138290; T-cell-specific surface glycoprotein CD28; Tp44 CD28 Tp44 CD28 molecule T-cell-specific surface glycoprotein CD28

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on CD28, check out the CD28 Infographic

CD28 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CD28: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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Order DetailsPrice
A00065-0.1 mg

0.1 mg

$449

Free Secondary Antibody

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Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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