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Product Info Summary
| SKU: | A00065 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-CD28 Antibody
SKU/Catalog Number
A00065
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-CD28 Antibody (Catalog # A00065). Tested in ELISA, WB, IHC, IF applications. This antibody reacts with Human.
Storage & Handling
CD28 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-CD28 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00065)
Host
Rabbit
Contents
CD28 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
CD28 antibody was raised against a peptide corresponding to 18 amino acids near the carboxy terminus of human CD28. The immunogen is located within the last 50 amino acids of CD28.
Reactive Species
A00065 is reactive to CD28 in Human
Observed Molecular Weight
68 kDa
Calculated molecular weight
25.1 kDa
Background of CD28
Estrogen receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of ligand-activated transcription factors (1). Estrogen receptors, including ESR1, also known as ER-alpha and ESR2 (ER-beta), contain DNA binding and ligand binding domains and are critically involved in regulating the normal function of reproductive tissues. ESR1 is a widely expressed nuclear protein and serves as a strong activator of estrogen responsive genes (1,2). Phosphorylation of serines 104 and 106, located in the N-terminal transcription activation function-1 domain (AF-1), plays a large role in regulating ER alpha activity (3).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00065 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
CD28 antibody can be used for Western blot at 1μg/mL. Antibody can also be used for Immunohistochemistry at 2 μg/mL. For Immunoflorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of CD28 in human spleen tissue lysate with CD28 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
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Immunohistochemistry of CD28 in human spleen tissue with CD28 antibody at 2 μg/ml.
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Immunofluorescence of CD28 in human spleen tissue with CD28 antibody at 20 μg/ml.
Specific Publications For Anti-CD28 Antibody (A00065)
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