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Product Info Summary
| SKU: | A02814 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-CD299 CLEC4M Antibody
SKU/Catalog Number
A02814
Size
100μl
Form
Liquid
Description
Boster Bio Anti-CD299 CLEC4M Antibody catalog # A02814. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD299 CLEC4M Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02814)
Host
Rabbit
Contents
PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthetic peptide from human protein at AA range: 271-320
Reactive Species
A02814 is reactive to CLEC4M in Human, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
45.4 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02814 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:500-200
ELISA 1:10000-20000
IF 1:50-200
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of mouse-lung mouse-lung lysate, antibody was diluted at 2000. Secondary antibody was diluted at 1:20000
Specific Publications For Anti-CD299 CLEC4M Antibody (A02814)
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Customer Q&As
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7 Customer Q&As for Anti-CD299 CLEC4M Antibody
Question
Is this A02814 anti-CD299 antibody reactive to the isotypes of CLEC4M?
Verified Customer
Verified customer
Asked: 2019-11-19
Answer
The immunogen of A02814 anti-CD299 antibody is Synthetic peptide from human protein at AA range: 271-320. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-11-19
Question
I see that the anti-CD299 antibody A02814 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-08-02
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-08-02
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for placenta testis using anti-CD299 antibody A02814. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-05-23
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-23
Question
We are currently using anti-CD299 antibody A02814 for mouse tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2018-08-21
Answer
The anti-CD299 antibody (A02814) has not been tested for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-08-21
Question
Please see the WB image, lot number and protocol we used for placenta testis using anti-CD299 antibody A02814. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-01-02
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-01-02
Question
Does anti-CD299 antibody A02814 work for WB with placenta testis?
A. Taylor
Verified customer
Asked: 2016-05-16
Answer
According to the expression profile of placenta testis, CLEC4M is highly expressed in placenta testis. So, it is likely that anti-CD299 antibody A02814 will work for WB with placenta testis.
Boster Scientific Support
Answered: 2016-05-16
Question
Will A02814 anti-CD299 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
S. Huang
Verified customer
Asked: 2013-04-10
Answer
It shows on the product datasheet, A02814 anti-CD299 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2013-04-10
