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Product Info Summary
| SKU: | A10132 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC |
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Product info
Product Name
Anti-CD300b CD300LB Antibody
SKU/Catalog Number
A10132
Size
100μl
Form
Liquid
Description
Boster Bio Anti-CD300b CD300LB Antibody catalog # A10132. Tested in IHC, IF, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD300b CD300LB Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10132)
Host
Rabbit
Contents
PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthetic peptide from human protein at AA range: 61-110
Reactive Species
A10132 is reactive to CD300LB in Human, Mouse
Calculated molecular weight
22.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A10132 is guaranteed for ELISA, IF, IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IHC 1:50-200
ELISA 1:10000-20000
IF 1:50-200
Validation Images & Assay Conditions
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Immunohistochemical analysis of paraffin-embedded human-liver-cancer, antibody was diluted at 1:200.
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Immunohistochemical analysis of paraffin-embedded human-liver-cancer, antibody was diluted at 1:200.
Specific Publications For Anti-CD300b CD300LB Antibody (A10132)
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5 Customer Q&As for Anti-CD300b CD300LB Antibody
Question
Can you help my question with product A10132, anti-CD300b antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-12-19
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A10132 anti-CD300b antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-12-19
Question
I see that the anti-CD300b antibody A10132 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-06-04
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-06-04
Question
I was wanting to use your anti-CD300b antibody for IHC for human leukocyte on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human leukocyte identification?
D. Jha
Verified customer
Asked: 2019-03-07
Answer
As indicated on the product datasheet, A10132 anti-CD300b antibody has been validated for IHC on human tissues. We have an innovator award program that if you test this antibody and show it works in human leukocyte in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-03-07
Question
I would like to test anti-CD300b antibody A10132 on human leukocyte for research purposes, then I may be interested in using anti-CD300b antibody A10132 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-05-24
Answer
The products we sell, including anti-CD300b antibody A10132, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-05-24
Question
We are currently using anti-CD300b antibody A10132 for human tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2017-11-17
Answer
The anti-CD300b antibody (A10132) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-11-17
