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Product Info Summary
| SKU: | A10652 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-CD300g CD300LG Antibody
SKU/Catalog Number
A10652
Size
100μl
Form
Liquid
Description
Boster Bio Anti-CD300g CD300LG Antibody catalog # A10652. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD300g CD300LG Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10652)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from the Internal region of human CD300LG. AA range:41-90
Reactive Species
A10652 is reactive to CD300LG in Human, Mouse
Calculated molecular weight
36.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A10652 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC: 1:100-300
ELISA 1:20000
IF 1:50-200
Validation Images & Assay Conditions
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Western Blot analysis of L929 cells using CD300g Polyclonal Antibody. Secondary antibody was diluted at 1:20000
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Western blot analysis of lysate from L929 cells, using CD300LG Antibody.
Specific Publications For Anti-CD300g CD300LG Antibody (A10652)
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4 Customer Q&As for Anti-CD300g CD300LG Antibody
Question
Will A10652 anti-CD300g antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-04-24
Answer
You can see on the product datasheet, A10652 anti-CD300g antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-04-24
Question
Do you have a BSA free version of anti-CD300g antibody A10652 available?
C. Mitchell
Verified customer
Asked: 2019-09-13
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CD300g antibody A10652 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-09-13
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for adipose tissue using anti-CD300g antibody A10652. Let me know if you need anything else.
R. Evans
Verified customer
Asked: 2015-10-22
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-10-22
Question
We are currently using anti-CD300g antibody A10652 for human tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on horse tissues as well?
T. Li
Verified customer
Asked: 2015-05-06
Answer
The anti-CD300g antibody (A10652) has not been tested for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-05-06
