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- Table of Contents
PECAM1 antibody (M01513-1) performs well in Flow Cytometry, IP, IHC, WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 3 Publications
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IHC analysis of CD31 using anti-CD31 antibody (M01513-1).
CD31 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-CD31 Antibody (M01513-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
CD31 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-CD31 Antibody (M01513-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Designing a Western blot for PECAM1? The guide covers the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.
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Anti-CD31 PECAM1 Rabbit Monoclonal Antibody
PECAM1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | Flow Cytometry, IP, IHC, WB |
Tested Applications
PECAM1 antibody was positively detected by WB in:
human placenta tissue, human Jurkat whole cell, human THP-1 whole cell, human HEL whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
PECAM1 antibody was positively detected by IHC in:
human placenta tissue, human liver cancer tissue
Use EDTA pH 8.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
WB 1:500-2000
IHC 1:50-200
IP 1:20
FC 1:20
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
IP
FC
Human
Tested
Tested
Recommend
Recommend
Recommend
Product Information
Product Name
Anti-CD31 PECAM1 Rabbit Monoclonal Antibody
View all PECAM1 Antibodies
View all PECAM1 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
CEE-16
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human CD31
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
120-130 kDa
Calculated Molecular Weight
82.5 kDa
Cite This Product
Anti-CD31 PECAM1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01513-1)
Protocols
Western Blot protocol for PECAM1 antibody
Immunohistochemistry (IHC) protocol for PECAM1 antibody
Publications
Application:
Species:
Synergistic Reversal of Inflammation-Mediated Degeneration in Intervertebral Discs: Phenylboric Acid-Grafted Hyaluronic Acid Hydrogel as an Anti-Oxidative Vehicle for Timp-3 Delivery and Promotion of Extracellular Matrix Synthesis
Published: 2025-06-06
Journal: Acta Biomaterialia
Application: IHC
Impact Factor: 9.60
Species: Rat
PMID: 40484295
The expressions of NGF and VEGF in the fracture tissues are closely associated with accelerated clavicle fracture healing in patients with traumatic brain injury
Published: 2022-12-26
Journal: Therapeutics and Clinical Risk Management
Application: IHC
Impact Factor: 2.76
Species: Human
PMID: 30538487
Distinct molecular basis for endothelial differentiation: Gene expression profiles of human mesenchymal stem cells versus umbilical vein endothelial cells
Published: 2014-02-02
Journal: CELLULAR IMMUNOLOGY
Application: FC
Impact Factor: 1.87
Species: Human
PMID: 24681515
Review
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Product Promise
We promise Anti-CD31 PECAM1 Rabbit Monoclonal Antibody performs excellently in relevant applications for Flow Cytometry, IP, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
M01513-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 19, 2026