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- Table of Contents
Epcam antibody (A00276-2) performs well in ELISA, Flow Cytometry, IF, IHC, WB and shows reactivity with Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Flow Cytometry analysis of RAW264.7 cells using anti-CD326/Epcam antibody (A00276-2).
Overlay histogram showing RAW264.7 cells stained with A00276-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CD326/Epcam Antibody (A00276-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Overlay histogram showing RAW264.7 cells stained with A00276-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CD326/Epcam Antibody (A00276-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Designing a Western blot for EPCAM? The guide covers the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.
Open the EPCAM Western Blot GuideBundle Save
Anti-CD326/Epcam Antibody Picoband®
Epcam antibody
| Reactive Species | Host | Application |
|---|---|---|
| Mouse, Rat | Rabbit | ELISA, Flow Cytometry, IF, IHC, WB |
Tested Applications
Epcam antibody was positively detected by WB in:
mouse small intestine tissue, mouse kidney tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Epcam antibody was positively detected by IHC in:
rat colon tissue, mouse colon tissue
Use EDTA pH 8.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Epcam antibody was positively detected by IF in:
mouse colon cancer tissue, rat colon cancer tissue
Epcam antibody was positively detected by FC in:
RAW2647 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.25 μg/ml
Mouse
Immunohistochemistry(Paraffin-embedded Section)
1-2 μg/ml
Mouse, Rat
Immunofluorescence
5 μg/ml
Mouse, Rat
Flow Cytometry (Fixed)
1-3 μg /1x106 cells
Mouse
ELISA
0.1-0.5 μg/ml
-
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
ELISA
Mouse
Tested
Tested
Tested
Recommend
Rat
Tested
Tested
Recommend
Product Information
Product Name
Anti-CD326/Epcam Antibody Picoband®
View all EPCAM Antibodies
View all EPCAM Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt.
After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months.
Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouse CD326/Epcam recombinant protein (Position: Q24-A267).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
38 kDa
Calculated Molecular Weight
35.0 kDa
Background of Epcam
Epithelial cell adhesion molecule (EpCAM) is a transmembrane glycoprotein mediating Ca2+-independent homotypic cell–cell adhesion in epithelia. This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy.
Cite This Product
Anti-CD326/Epcam Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00276-2)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Mouse
100%
High Homology
Rat
90%
High Homology
Monkey
83%
Moderate Homology
Rabbit
83%
Moderate Homology
Human
82%
Moderate Homology
Sheep
80%
Moderate Homology
Cow
80%
Moderate Homology
Dog
80%
Moderate Homology
Zebrafish
42%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for Epcam antibody
Immunohistochemistry (IHC) protocol for Epcam antibody
Immunofluorescence (IF) protocol for Epcam antibody
Flow Cytometry (FC) protocol for Epcam antibody
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Product Promise
We promise Anti-CD326/Epcam Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, IF, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A00276-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 30, 2026