Product Info Summary
| SKU: | A01508 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-CD33 Antibody
SKU/Catalog Number
A01508
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-CD33 Antibody (Catalog # A01508). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
CD33 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-CD33 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01508)
Host
Rabbit
Contents
CD33 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
CD33 antibody was raised against a 14 amino acid synthetic peptide near the center of human CD33. The immunogen is located within amino acids 180 - 230 of CD33.
Cross-reactivity
At least three isoforms of CD33 are known to exist; this antibody will detect all three isoforms.
Reactive Species
A01508 is reactive to CD33 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
39.8 kDa
Background of CD33
CD33 is a member of the sialic acid-binding immunoglobulin-like lectin (Siglec) family that is highly expressed on myeloid progenitor cells. Assessment of CD33 expression is of great importance in the immunodiagnosis of acute leukemia, allowing distinction between myeloid and lymphoid origin, as CD33 is generally restricted to the myelomonocytic lineage. CD33 can associate with the protein-tyrosine phosphatases SHP-1 and SHP-2 and thus could modulate downstream signaling events associated with cell activation. Common variants of CD33 have been found to be associated with late-onset Alzheimer's disease.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01508 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
CD33 antibody can be used for detection of CD33 by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of CD33 in 3T3 cell lysate with CD33 antibody at (A) 1 and (B) 2 μg/mL.
Specific Publications For Anti-CD33 Antibody (A01508)
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