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Product Info Summary
| SKU: | M00885 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-CD34 Antibody (C-term)
SKU/Catalog Number
M00885
Size
80 µl
Description
Boster Bio Anti-CD34 Antibody (C-term) (Catalog # M00885). Tested in Flow Cytometry, WB application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-CD34 Antibody (C-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00885)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This CD34 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 356-390 amino acids from the C-terminal region of human CD34.
Reactive Species
M00885 is reactive to CD34 in Human
Calculated molecular weight
40.7 kDa
Background of CD34
Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00885 is guaranteed for Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
FC: 1:25
Validation Images & Assay Conditions
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Western blot analysis of lysate from KG-1 cell line, using CD34 Antibody (C-term). M00885 was diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.
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Flow cytometric analysis of HT-29 cells using CD34 Antibody (C-term) (green, Cat#) compared to an isotype control of rabbit IgG (blue). was diluted at 1:25 dilution. An Alexa Fluor® 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
Specific Publications For Anti-CD34 Antibody (C-term) (M00885)
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