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Cd34 antibody (A00885-1) performs well in ELISA, IHC, WB and shows reactivity with Mouse, Rat.

Validated for inter-lot consistency, specificity, and reproducibility
Committed to quality since 1993
Over 40 Publications

a00885 1 cd34 primary antibodies ihc testing 2

IHC analysis of CD34 using anti-CD34 antibody (A00885-1).
CD34 was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-CD34 Antibody (A00885-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

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Anti-CD34 Antibody Picoband®

Cd34 antibody

40 Publications

Reactive Species	Host	Application
Mouse, Rat	Rabbit	ELISA, IHC, WB

Tested Applications

Cd34 antibody was positively detected by WB in:

rat heart tissue, rat skeletal muscle tissue, rat lung tissue, mouse lung tissue

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

Cd34 antibody was positively detected by IHC in:

rat brain tissue, rat kidney tissue

Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.

Reactive Data

Boster Guarantee: All Tested Recommend Predicted species What is this?

IHC

ELISA

Rat

Tested

Recommend

Mouse

Tested

Recommend

Product Information

Product Name

Anti-CD34 Antibody Picoband®
View all CD34 Antibodies

Form

Lyophilized

Uniprot

B1PLB1

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived rat CD34 recombinant protein (Position: S170-L386).

Cross-Reactivity

No cross-reactivity with other proteins.

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

105 kDa

Background of Cd34

CD34 is a monomeric cell surface antigen with a molecular mass of approximately 110 KD.CD34 is expressed in humans in hematopoietic stem cells, vascular endothelium, and blasts from 30% of patients with acute myeloid and lymphocytic leukemia. The human CD34 gene spans 26 kb and has 8 exons, a structure quite similar to that of the murine gene. By Southern blot analysis of DNA from a panel of human x mouse somatic cell hybrids using a CD34 cDNA probe demonstrate that the gene for CD34 is located on human chromosome 1 in the 1q12----qter region. CD34 plays an important role in the formation of progenitor cells during both embryonic and adult hematopoiesis.

Cite This Product

Anti-CD34 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00885-1)

Protocols

Western Blot protocol for Cd34 antibody

Review Protocol

Immunohistochemistry (IHC) protocol for Cd34 antibody

Review Protocol

Publications

Apelin-13 regulated thrombotic inflammation and induced homing of EPCs to promote the dissolution and recanalization of deep vein thrombosis

Published: 2025-12-13

Journal: INTERNATIONAL IMMUNOPHARMACOLOGY

Application: FC

Impact Factor: 4.70

Species: Rat

PMID: 41391282

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Anti-CD34 Antibody Picoband®

Cd34 antibody

Overview

Tested Applications

Recommend Dilution

Reactive Data

Product Information

Protocols

Publications

Apelin-13 regulated thrombotic inflammation and induced homing of EPCs to promote the dissolution and recanalization of deep vein thrombosis

Effects of norepinephrine‑induced activation of rat vascular adventitial fibroblasts on proliferation and migration of BMSCs involved in vascular remodeling

Cancer-Associated Fibroblasts Regulate Bladder Cancer Invasion and Metabolic Phenotypes through Autophagy

New lymphatic cell formation is associated with damaged brain tissue clearance after penetrating traumatic brain injury

Survivable potential of germ cells after trehalose cryopreservation of bovine testicular tissues

Curcumin Derivative Cur20 Attenuated Cerebral Ischemic Injury by Antioxidant Effect and HIF-1α/VEGF/TFEB-Activated Angiogenesis

Secretoneurin, a Neuropeptide, Enhances Bone Regeneration in a Mouse Calvarial Bone Defect Model

Effect of chitosan oligosaccharide-conjugated selenium on improving immune function and blocking gastric cancer growth

Possibilities and limitations of electrospun chitosan-coated polycaprolactone grafts for rotator cuff tear repair

Antitumor evaluation of carboxymethyl chitosan based norcantharidin conjugates against gastric cancer as novel polymer therapeutics

piRNA-823 delivered by multiple myeloma-derived extracellular vesicles promoted tumorigenesis through re-educating endothelial cells in the tumor environment

Long noncoding RNA HOTAIR silencing inhibits invasion and proliferation of human colon cancer LoVo cells via regulating IGF2BP2

Effects of CD100 promote wound healing in diabetic mice

Involvement of aberrantly activated HOTAIR/EZH2/miR-193a feedback loop in progression of prostate cancer

Metastatic hemangiosarcoma of the liver in a young rat

Ginkgo biloba exocarp extracts inhibits angiogenesis and its effects on Wnt/β-catenin-VEGF signaling pathway in Lewis lung cancer

The effects of ethanol on angiogenesis after myocardial infarction, and preservation of angiogenesis with rosuvastatin after heavy drinking

Arginine ADP-ribosyltransferase 1 promotes angiogenesis in colorectal cancer via the PI3K/Akt pathway

Andrographolide inhibits melanoma tumor growth by inactivating the TLR4/NF-κB signaling pathway

Effect of angiogenesis inhibitor SU6668 in combination with 5-Fu on liver metastasis from transplantation tumors of human colorectal cancer in nude mice

Crotoxin suppresses the tumorigenic properties and enhances the antitumor activity of Iressa® (gefinitib) in human lung adenocarcinoma SPCA‑1 cells

Effects of all‑trans retinoic acid on VEGF and HIF‑1α expression in glioma cells under normoxia and hypoxia and its anti‑angiogenic effect in an intracerebral glioma model

Activated Hair Follicle Stem Cells and Wnt/β-catenin Signaling Involve in Pathnogenesis of Sebaceous Neoplasms

Comparison of administration routes for adipose-derived stem cells in the treatment of middle cerebral artery occlusion in rats

Effects of BMSCs interactions with adventitial fibroblasts in transdifferentiation and ultrastructure processes

A novel decoy receptor fusion protein for FGF-2 potently inhibits tumour growth

Modulating hair follicle size with Wnt10b/DKK1 during hair regeneration

Magnetic fluid hyperthermia inhibits the growth of breast carcinoma and downregulates vascular endothelial growth factor expression

Preparation, antiangiogenic and antitumoral activities of the chemically sulfated glucan from Phellinus ribis

The Renoprotective Effect of Bone Marrow-Derived Endothelial Progenitor Cell Transplantation on Acute Ischemia-Reperfusion Injury in Rats

Regression effect of hepatocyte nuclear factor 4α on liver cirrhosis in rats

Histopathological studies of microtubule disassembling agent-induced myocardial lesions in rats

Antitumor activity of Endostar combined with radiation against human nasopharyngeal carcinoma in mouse xenograft models

Effects of total flavonoids from Drynaria fortunei on the proliferation and osteogenic differentiation of rat dental pulp stem cells

Inhibition of STAT3 by RNA interference suppresses angiogenesis in colorectal carcinoma

In Vivo Serial MR Imaging of Magnetically Labeled Endothelial Progenitor Cells Homing to the Endothelium Injured Artery in Mice

Effect of Fluid Shear Stress on Cardiomyogenic Differentiation of Rat Bone Marrow Mesenchymal Stem Cells

Beta-elemene inhibits melanoma growth and metastasis via suppressing vascular endothelial growth factor-mediated angiogenesis

Antitumor activity of an adenovirus harboring human IL-24 in colon cancer

Estrogen-replacement therapy promotes angiogenesis after acute myocardial infarction by enhancing SDF-1 and estrogen receptor expression

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