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Product Info Summary
| SKU: | A02421 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-CD3Z Antibody (C-term)
SKU/Catalog Number
A02421
Size
80 µl
Description
Boster Bio Anti-CD3Z Antibody (C-term) (Catalog # A02421). Tested in WB, Flow Cytometry application(s). This antibody reacts with Human, Mouse.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-CD3Z Antibody (C-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02421)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This CD3Z antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 113-141 amino acids from the C-terminal region of human CD3Z.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A02421 is reactive to CD247 in Human, Mouse
Reconstitution
Calculated molecular weight
18696 Da
Background of CD247
T-cell receptor zeta, together with T-cell receptor alpha/beta and gamma/delta heterodimers, and with CD3-gamma, -delta and -epsilon, forms the T-cell receptor-CD3 complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. Low expression of the antigen results in impaired immune response.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02421 is guaranteed for Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:4000-1:8000
FC: 1:25
Validation Images & Assay Conditions
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Western blot analysis of CD3Z (arrow) using rabbit polyclonal CD3Z Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CD3Z gene (Lane 2) (Origene Technologies).
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All lanes : Anti-CD3Z Antibody (C-term) at 1:4000-1:8000 dilution
Lane 1: Jurkat whole cell lysate
Lane 2: MOLT-4 whole cell lysate
Lane 3: Mouse thymus lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 19 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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Overlay histogram showing K562 cells stained with A02421 (green line). The cells were fixed with 2% paraformaldehyde (10 min). The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (A02421, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Protein Target Info & Infographic
Gene/Protein Information For CD247 (Source: Uniprot.org, NCBI)
Gene Name
CD247
Full Name
T-cell surface glycoprotein CD3 zeta chain
Weight
18696 Da
Superfamily
CD3Z/FCER1G family
Alternative Names
T-cell surface glycoprotein CD3 zeta chain, T-cell receptor T3 zeta chain, CD247, CD247, CD3Z, T3Z, TCRZ CD247 CD3-ZETA, CD3H, CD3Q, CD3Z, IMD25, T3Z, TCRZ CD247 molecule T-cell surface glycoprotein CD3 zeta chain|CD247 , zeta subunit|CD3Z , zeta polypeptide (TiT3 complex)|CD3zeta chain|T-cell receptor complex, zeta subunit of CD3|T-cell receptor T3 zeta chain|TCR zeta chain
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD247, check out the CD247 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD247: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD3Z Antibody (C-term) (A02421)
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