Anti-CD4 Antibody (Monoclonal, 35C63)

Product Name

Anti-CD4 Antibody (Monoclonal, 35C63)

View all Cd4 Antibodies

SKU/Catalog Number

M00344-11
BM0212 is an alternative SKU for this antibody, used in previous lots.

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-CD4 Antibody (Monoclonal, 35C63) catalog # M00344-11. Tested in Flow Cytometry, IHC, ICC applications. This antibody reacts with Human.

CD4 is a T-cell surface glycoprotein that functions primarily as an MHC class II coreceptor, supporting TCR signaling and defining CD4+ T-cell lineages (canonical). Assay context: Human-reactive monoclonal validated for Flow Cytometry, IHC, and ICC; immunophenotyping panels commonly interpret CD4 frequency/localization alongside effector function readouts such as GZMB (putative) and anti-inflammatory cytokines such as IL10 (cross-context; putative).

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CD4 Antibody (Monoclonal, 35C63) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00344-11)

Host

Mouse

Contents

Mouse IgG in stabilizing components, 1.2% sodium acetate and 0.01mg NaN3.

Clonality

Monoclonal

Clone Number

35C63

Isotype

Mouse IgG1

Immunogen

CD4-transfected mouse T-cell hybridoma, 3DT, followed by CD4+ human T-cell CEM cells.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

M00344-11 is reactive to Cd4 in Human

Calculated molecular weight

51.4 kDa

Background of Cd4

CD stands for "cluster of differentiation"; the number that follows is arbitrarily assigned. In the full designation the cell type and nature and molecular weight of the antigen are given in brackets; for CD4, this is as follows: [T,gp55]. CD4 is present on a subset of T lymphocytes ("helper/inducer" T cells) and is also expressed at a lower level on monocytes, tissue macrophages and granulocytes. The antigen is involved in binding to MHC class II molecules. The intracellular domain of the antigen is associated with p56lck protein tyrosine kinase.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M00344-11 is guaranteed for Flow Cytometry, IHC, ICC Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Frozen Section), 1μg/ml, Human
Immunocytochemistry, 0.5-1μg/ml, Human
Flow Cytometry, 1-3μg/1x10⁶ cells, Human

Validation Images & Assay Conditions

There are currently no images for this product. We are working on uploading them please check back shortly or contact us to expedite our upload process for this antibody.

See full target information Cd4

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