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- Table of Contents
CD4 antibody (M00344-2) performs well in ELISA, IHC-P, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Immunohistochemistry Validation of CD4 in Human Thymus Tissue
Immunohistochemical analysis of paraffin-embedded human thymus tissue using anti-CD4 antibody (M00344-2) at 5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-mouse IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Immunohistochemical analysis of paraffin-embedded human thymus tissue using anti-CD4 antibody (M00344-2) at 5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-mouse IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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Anti-CD4 Monoclonal Antibody [9H5A8]
CD4 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Mouse | ELISA, IHC-P, WB |
Recommend Dilution
Application
Dilution
Species
WB: 0.5-4 μg/mL; IHC: 5 μg/mL.
Antibody validated: Western Blot in human
mouse and rat samples; Immunohistochemistry in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-CD4 Monoclonal Antibody [9H5A8]
View all CD4 Antibodies
View all CD4 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
CD4 monoclonal antibody can be stored at 4°C, stable for one year.
Host
Mouse
Contents
CD4 Monoclonal Antibody is supplied in PBS containing ≤0.09% sodium azide.
Clonality
Monoclonal
Clone Number
Clone: 9H5A8
Isotype
IgG1
Immunogen
Mouse monoclonal CD4 antibody was raised against a 193 amino acid recombinant protein from near the amino terminus of human CD4.
Observed Molecular Weight
68 kDa
Calculated Molecular Weight
51.1 kDa
Background of CD4
CD4 is a member of the immunoglobulin superfamily and is implicated as associative recognition element in MHC (Major Histocompatibility Complex) class II-restricted immune response. On T-lymphocytes, it defines the helper/inducer subset. The mature 55 kd CD4 protein consists of a 372 amino acid extracellular segment composed of four tandem immunoglobulin-like VJ regions. The CD4 molecule is a major receptor for human immunodeficiency virus (HIV), binding directly to the envelope glycoprotein gp120 on HIV, with the co-receptors being CCR5 or CXCR4. It has been shown that the V-like domains are critical for binding with HIV envelope gp120.
Cite This Product
Anti-CD4 Monoclonal Antibody [9H5A8] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00344-2)
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Product Promise
We promise Anti-CD4 Monoclonal Antibody [9H5A8] performs excellently in relevant applications for ELISA, IHC-P, WB. If it doesn't perform as promised, we will refund the product. Learn more
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M00344-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$481.00
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 11, 2026