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Product Info Summary
| SKU: | M00344-2 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | ELISA, IHC-P, WB |
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Product info
Product Name
Anti-CD4 Monoclonal Antibody [9H5A8]
SKU/Catalog Number
M00344-2
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-CD4 Monoclonal Antibody [9H5A8] (Catalog # M00344-2). Tested in ELISA, WB, IHC-P applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
CD4 monoclonal antibody can be stored at -20°C, stable for one year.
Cite This Product
Anti-CD4 Monoclonal Antibody [9H5A8] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00344-2)
Host
Mouse
Contents
CD4 Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Monoclonal
Clone Number
Clone: 9H5A8
Isotype
IgG1
Immunogen
Mouse monoclonal CD4 antibody was raised against a 193 amino acid recombinant protein from near the amino terminus of human CD4.
Reactive Species
M00344-2 is reactive to CD4 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
51.1 kDa
Background of CD4
CD4 is a member of the immunoglobulin superfamily and is implicated as associative recognition element in MHC (Major Histocompatibility Complex) class II-restricted immune response. On T-lymphocytes, it defines the helper/inducer subset. The mature 55 kd CD4 protein consists of a 372 amino acid extracellular segment composed of four tandem immunoglobulin-like VJ regions. The CD4 molecule is a major receptor for human immunodeficiency virus (HIV), binding directly to the envelope glycoprotein gp120 on HIV, with the co-receptors being CCR5 or CXCR4. It has been shown that the V-like domains are critical for binding with HIV envelope gp120.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00344-2 is guaranteed for ELISA, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 0.5-4 μg/mL; IHC: 5 μg/mL.
Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western Blot Validation with Human Recombinant Protein
Loading: 5ng (Lane 1), 25ng (Lane 2) and 100ng (Lane 3) of human CD4 recombinant protein.
Antibodies: CD4 M00344-2 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution.
Observed at around 27kD.
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Western Blot Validation in Human Thymus Tissue Lysate
Loading: 15 μg of lysates per lane.
Antibodies: CD4 M00344-2 (Lane 1: 0.5 μg/mL, Lane 2: 1 μg/mL and Lane 3: 2 μg/mL), 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution.
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Western Blot Validation in Human Jurkat Lysate
Loading: 15 μg of lysates per lane.
Antibodies: CD4 M00344-2 (Lane 1: 1 μg/mL, Lane 2: 2 μg/mL and Lane 3: 4 μg/mL), 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution.
Click image to see more details
Western Blot Validation in Human, Mouse and Rat Tissue Lysate
Loading: 15 μg of lysates per lane.
Antibodies: CD4 M00344-2 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution.
Click image to see more details
Western Blot Validation in Human Cell Lines
Loading: 15 μg of lysates per lane.
Antibodies: CD4 M00344-2 (4 μg/mL), 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution.
Click image to see more details
Western Blot Validation in Mouse Spleen Tissue Lysate
Loading: 15 μg of lysates per lane.
Antibodies: CD4 M00344-2 (Lane 1: 1 μg/mL, Lane 2: 2 μg/mL and Lane 3: 4 μg/mL), 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution.
Click image to see more details
Western Blot Validation in Rat Lung Tissue Lysate
Loading: 15 μg of lysates per lane.
Antibodies: CD4 M00344-2 (Lane 1: 0.5 μg/mL, Lane 2: 1 μg/mL and Lane 3: 2 μg/mL), 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution.
Click image to see more details
Immunohistochemistry Validation of CD4 in Human Thymus Tissue
Immunohistochemical analysis of paraffin-embedded human thymus tissue using anti-CD4 antibody (M00344-2) at 5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-mouse IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Specific Publications For Anti-CD4 Monoclonal Antibody [9H5A8] (M00344-2)
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