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Cd4 antibody (A00344-3) performs well in ELISA, IHC and shows reactivity with Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 4 Publications
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IHC analysis of Cd4 using anti-Cd4 antibody (A00344-3).
Cd4 was detected in a paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Cd4 Antibody (A00344-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Cd4 was detected in a paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Cd4 Antibody (A00344-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Tested Applications
Cd4 antibody was positively detected by IHC in:
rat spleen tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml
Rat
ELISA
0.1-0.5 μg/ml
Rat
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-Cd4 Antibody
View all CD4 Antibodies
View all CD4 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived rat Cd4 recombinant protein (Position: K28-A246).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Calculated Molecular Weight
51.4 kDa
Background of Cd4
This gene encodes the CD4 membrane glycoprotein of T lymphocytes. The CD4 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class II MHC molecules. The CD4 antigen is also a primary receptor for entry of the human immunodeficiency virus through interactions with the HIV Env gp120 subunit. This gene is expressed not only in T lymphocytes, but also in B cells, macrophages, granulocytes, as well as in various regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been identified in this gene.
Cite This Product
Anti-Cd4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00344-3)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Rat
100%
High Homology
Mouse
70%
Moderate Homology
Rabbit
50%
Low homology
Human
49%
Low homology
Monkey
48%
Low homology
Sheep
47%
Low homology
Cow
46%
Low homology
Dog
42%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Immunohistochemistry (IHC) protocol for Cd4 antibody
Publications
Application:
Species:
Treating cutaneous squamous cell carcinoma using 5-aminolevulinic acid polylactic-co-glycolic acid nanoparticle-mediated photodynamic therapy in a mouse model
Published: 2023-02-27
Journal: International Journal of Nanomedicine
Application: IHC
Impact Factor: 8.00
Species: Mouse
PMID: 25609949
Interleukin-15 gene therapy and the mammalian target of rapamycin inhibitor everolimus inhibit the growth of metastatic breast cancer
Published: 2013-09-03
Journal: JOURNAL OF GENE MEDICINE
Application: IHC
Impact Factor: 2.16
Species: Mouse
PMID: 24038990
Endocrine function and lymphocyte infiltration of newborn rat ovaries after ultrarapid freezing and allotransplantation
Published: 2007-02-12
Journal: FERTILITY AND STERILITY
Application: IHC
Impact Factor: 3.28
Species: Rat
PMID: 17296191
Induction of immune tolerance with heart-thymus composite allotransplantation in rats
Published: 2005-10-01
Journal: JOURNAL OF CENTRAL SOUTH UNIVERSITY OF TECHNOLOGY
Impact Factor: 0.19
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Product Promise
We promise Anti-Cd4 Antibody performs excellently in relevant applications for ELISA, IHC. If it doesn't perform as promised, we will refund the product. Learn more
In stock
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A00344-3
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 15, 2026