Anti-CD47 Antibody Picoband™

Boster Bio Anti-CD47 Antibody Picoband™ catalog # A00360-2. Tested in ELISA, Flow Cytometry, IF, IHC-P, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A00360-2
Size: 100μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, Flow Cytometry, IF, IHC-P, IHC-F, ICC, WB

Product Name

Anti-CD47 Antibody Picoband™

See all CD47 products

SKU/Catalog Number

A00360-2

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-CD47 Antibody Picoband™ catalog # A00360-2. Tested in ELISA, Flow Cytometry, IF, IHC-P, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CD47 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00360-2)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

E.coli-derived human CD47 recombinant protein (Position: Q19-E323).

Cross Reactivity

No cross reactivity with other proteins.

Reactive Species

A00360-2 is reactive to CD47 in Human, Mouse, Rat

Applications

A00360-2 is guaranteed for ELISA, Flow Cytometry, IF, IHC-P, IHC-F, ICC, WB Boster Guarantee

*Blocking peptide can be purchased at $150. Contact us for more information.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Immunohistochemistry (Frozen Section), 0.5-1μg/ml
Immunocytochemistry/Immunofluorescence, 2μg/ml
Flow Cytometry, 1-3μg/1x106
Direct ELISA, 0.1-0.5μg/ml

Validation Images & Assay Conditions

Gene/Protein Information For CD47 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q08722

Gene ID

961

Gene Name

CD47

Full Name

Leukocyte surface antigen CD47

Weight

35.214kDa

Alternative Names

antigen identified by monoclonal 1D8; Antigenic surface determinant protein OA3; CD47 antigen (Rh-related antigen, integrin-associated signal transducer); CD47 antigen; CD47 glycoprotein; CD47 molecule; CD47; IAP; IAPintegrin associated protein; Integrin-associated protein; leukocyte surface antigen CD47; MER6integrin-associated signal transducer; OA3; Protein MER6; Rh-related antigen CD47 IAP, MER6, OA3 CD47 molecule leukocyte surface antigen CD47|CD47 antigen (Rh-related antigen, integrin-associated signal transducer)|CD47 glycoprotein|Rh-related antigen|antigen identified by monoclonal antibody 1D8|antigenic surface determinant protein OA3|integrin associated protein|integrin-associated signal transducer

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CD47, check out the CD47 Infographic

CD47 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CD47: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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5 Customer Q&As for Anti-CD47 Antibody Picoband™

Question

We have seen staining in mouse ovary. What should we do? Is anti-CD47 antibody supposed to stain ovary positively?

Verified Customer

Verified customer

Asked: 2020-04-17

Answer

Based on literature ovary does express CD47. Based on Uniprot.org, CD47 is expressed in visceral pleura, ovary, myelomonocyte, brain, hippocampus, leiomyosarcoma ovarian adenocarcinoma, erythrocyte, t-cell, liver, leukemic t-cell, among other tissues. Regarding which tissues have CD47 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 14702039
Erythrocyte, Pubmed ID: 7998989
Hippocampus, Leiomyosarcoma, and Ovarian adenocarcinoma, Pubmed ID: 15489334
Leukemic T-cell, Pubmed ID: 19349973
Liver, Pubmed ID: 19159218
Myelomonocyte, Pubmed ID: 7691831
Ovary, Pubmed ID: 1394148
T-cell, Pubmed ID: 15383453

Boster Scientific Support

Answered: 2020-04-17

Question

We were well pleased with the WB result of your anti-CD47 antibody. However we have observed positive staining in ovary cell membrane using this antibody. Is that expected? Could you tell me where is CD47 supposed to be expressed?

A. Baker

Verified customer

Asked: 2019-10-29

Answer

According to literature, ovary does express CD47. Generally CD47 expresses in cell membrane. Regarding which tissues have CD47 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 14702039
Erythrocyte, Pubmed ID: 7998989
Hippocampus, Leiomyosarcoma, and Ovarian adenocarcinoma, Pubmed ID: 15489334
Leukemic T-cell, Pubmed ID: 19349973
Liver, Pubmed ID: 19159218
Myelomonocyte, Pubmed ID: 7691831
Ovary, Pubmed ID: 1394148
T-cell, Pubmed ID: 15383453

Boster Scientific Support

Answered: 2019-10-29

Question

Our lab used your anti-CD47 antibody for WB on visceral pleura last year. I am using mouse, and We want to use the antibody for ELISA next. I am looking for examining visceral pleura as well as hippocampus in our next experiment. Could you please give me some suggestion on which antibody would work the best for ELISA?

Verified Customer

Verified customer

Asked: 2019-07-09

Answer

I have checked the website and datasheets of our anti-CD47 antibody and it seems that A00360-2 has been validated on mouse in both WB and ELISA. Thus A00360-2 should work for your application. Our Boster satisfaction guarantee will cover this product for ELISA in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for ELISA detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2019-07-09

Question

We are currently using anti-CD47 antibody A00360-2 for mouse tissue, and we are well pleased with the Flow Cytometry results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on canine tissues as well?

M. Yang

Verified customer

Asked: 2017-12-20

Answer

The anti-CD47 antibody (A00360-2) has not been tested for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2017-12-20

Question

I am interested in using your anti-CD47 antibody for positive regulation of stress fiber assembly studies. Has this antibody been tested with western blotting on u937 cells? We would like to see some validation images before ordering.

P. Gonzalez

Verified customer

Asked: 2013-08-12

Answer

Thank you for your inquiry. This A00360-2 anti-CD47 antibody is tested on rat kidney tissue, brain tissue, mouse brain, human tonsil tissue, ovary cancer tissue, u937 cells. It is guaranteed to work for ELISA, Flow Cytometry, IF, IHC-P, IHC-F, ICC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2013-08-12

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A00360-2
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$280.00

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