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Product Info Summary
| SKU: | M03484 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Mouse |
| Host: | Rabbit |
| Application: | IP, WB |
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Product info
Product Name
Anti-CD52 Monoclonal Antibody
SKU/Catalog Number
M03484
BM5629 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-CD52 Monoclonal Antibody catalog # M03484. Tested in WB, IP applications. This antibody reacts with Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD52 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03484)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AEIF-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human CD52
Reactive Species
M03484 is reactive to Cd52 in Mouse
Observed Molecular Weight
15-20 kDa
Calculated molecular weight
7.8 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03484 is guaranteed for IP, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IP 1:50
Positive Control
WB: Mouse spleen cell
Validation Images & Assay Conditions
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Western blot analysis of CD52 expression in Mouse spleen cell lysate.
Specific Publications For Anti-CD52 Monoclonal Antibody (M03484)
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3 Customer Q&As for Anti-CD52 Monoclonal Antibody
Question
We are currently using anti-CD52 Monoclonal antibody M03484 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says mouse. Is it true that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2019-09-19
Answer
The anti-CD52 Monoclonal antibody (M03484) has not been validated for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-19
Question
Does M03484 anti-CD52 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
B. Parker
Verified customer
Asked: 2018-05-22
Answer
As indicated on the product datasheet, M03484 anti-CD52 Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-05-22
Question
Will anti-CD52 Monoclonal antibody M03484 work on horse IP with spleen?
W. Jones
Verified customer
Asked: 2014-12-16
Answer
Our lab technicians have not validated anti-CD52 Monoclonal antibody M03484 on horse. You can run a BLAST between horse and the immunogen sequence of anti-CD52 Monoclonal antibody M03484 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse spleen in IP, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-12-16
