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Product Info Summary
| SKU: | M00910-1 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | WB |
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Product info
Product Name
Anti-CD55 Antibody
SKU/Catalog Number
M00910-1
Size
80 µl
Description
Boster Bio Anti-CD55 Antibody (Catalog # M00910-1). Tested in WB application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-CD55 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00910-1)
Host
Mouse
Contents
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Monoclonal
Clone Number
561CT4.2.3
Isotype
IgG1
Immunogen
This CD55 antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 51-79 amino acids from human CD55.
Reactive Species
M00910-1 is reactive to CD55 in Human
Calculated molecular weight
41.4 kDa
Background of CD55
This gene encodes a protein involved in the regulation of the complement cascade. The encoded glycoprotein is also known as the decay-accelerating factor (DAF); binding of DAF to complement proteins accelerates their decay, disrupting the cascade and preventing damage to host cells. Antigens present on the DAF glycoprotein constitute the Cromer blood group system (CROM). Two alternatively spliced transcripts encoding different proteins have been identified. The predominant transcript encodes a membrane-bound protein expressed on cells exposed to plasma component proteins but an alternatively spliced transcript produces a soluble protein present at much lower levels. Additional, alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq].
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00910-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:100
Validation Images & Assay Conditions
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Anti-CD55 Antibody (N-term) at 1:2000 dilution + Hela whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 41 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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CD55 Antibody western blot analysis in Hela cell line lysates (35μg/lane).This demonstrates the CD55 antibody detected the CD55 protein (arrow).
Specific Publications For Anti-CD55 Antibody (M00910-1)
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