Product Info Summary
SKU: | A02853 |
---|---|
Size: | 100 µl |
Reactive Species: | Human |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-CD70/Cd27L Antibody
View all CD27 Ligand/TNFSF7/CD70 Antibodies
SKU/Catalog Number
A02853
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-CD70/Cd27L Antibody (Catalog # A02853). Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD70/Cd27L Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02853)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human CD70.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A02853 is reactive to CD70 in Human
Reconstitution
Calculated molecular weight
21118 MW
Background of CD27 Ligand/TNFSF7/CD70
Cytokine that binds to CD27. Plays a role in T-cell activation. Induces the proliferation of costimulated T-cells and enhances the generation of cytolytic T-cells.
Beckmann M.P., Cell 73:447-456(1993).
Herrmann S., J. Immunol. 152:1756-1761(1994).
The MGC Project Team, Genome Res. 14:2121-2127(2004).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02853 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of extracts from HuvEC, K562, HepG2, Jurkat, HeLa and COLO205 cells, using CD70 antibody A02853.
Protein Target Info & Infographic
Gene/Protein Information For CD70 (Source: Uniprot.org, NCBI)
Gene Name
CD70
Full Name
CD70 antigen
Weight
21118 MW
Superfamily
tumor necrosis factor family
Alternative Names
CD70 antigen;CD27 ligand;CD27-L;Tumor necrosis factor ligand superfamily member 7;CD70;CD70;CD27L, CD27LG, TNFSF7; CD70 CD27-L, CD27L, CD27LG, LPFS3, TNFSF7, TNLG8A CD70 molecule CD70 |CD27 ligand|Ki-24 |surface CD70|tumor necrosis factor ligand 8A|tumor necrosis factor ligand superfamily member 7
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD70, check out the CD70 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD70: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD70/Cd27L Antibody (A02853)
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No publications found for A02853
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Customer Q&As
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7 Customer Q&As for Anti-CD70/Cd27L Antibody
Question
My question regarding product A02853, anti-CD70/Cd27L antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-08-27
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A02853 anti-CD70/Cd27L antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-08-27
Question
We are currently using anti-CD70/Cd27L antibody A02853 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-04
Answer
The anti-CD70/Cd27L antibody (A02853) has not been validated for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-04
Question
See attached the WB image, lot number and protocol we used for b-cell using anti-CD70/Cd27L antibody A02853. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-06-27
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-27
Question
Will anti-CD70/Cd27L antibody A02853 work for WB with b-cell?
Verified Customer
Verified customer
Asked: 2018-10-30
Answer
According to the expression profile of b-cell, CD70 is highly expressed in b-cell. So, it is likely that anti-CD70/Cd27L antibody A02853 will work for WB with b-cell.
Boster Scientific Support
Answered: 2018-10-30
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for b-cell using anti-CD70/Cd27L antibody A02853. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-08-03
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-08-03
Question
Is this A02853 anti-CD70/Cd27L antibody reactive to the isotypes of CD70?
Verified Customer
Verified customer
Asked: 2017-09-13
Answer
The immunogen of A02853 anti-CD70/Cd27L antibody is Synthesized peptide derived from internal of human CD70. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-09-13
Question
Our lab want to know about to test anti-CD70/Cd27L antibody A02853 on human b-cell for research purposes, then I may be interested in using anti-CD70/Cd27L antibody A02853 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-05-26
Answer
The products we sell, including anti-CD70/Cd27L antibody A02853, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-05-26